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Testis single-cell RNA-seq reveals the dynamics of de novo gene transcription and germline mutational bias in Drosophila

Evan Witt, Sigi Benjamin, Nicolas Svetec, Li Zhao
doi: https://doi.org/10.1101/689687
Evan Witt
1Laboratory of Evolutionary Genetics and Genomics, The Rockefeller University, New York, NY 10065, USA
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Sigi Benjamin
1Laboratory of Evolutionary Genetics and Genomics, The Rockefeller University, New York, NY 10065, USA
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Nicolas Svetec
1Laboratory of Evolutionary Genetics and Genomics, The Rockefeller University, New York, NY 10065, USA
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Li Zhao
1Laboratory of Evolutionary Genetics and Genomics, The Rockefeller University, New York, NY 10065, USA
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  • For correspondence: lzhao@rockefeller.edu
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Summary

The testis is a peculiar tissue in many respects. It shows patterns of rapid gene evolution and provides a hotspot for the origination of genetic novelties such as de novo genes, duplications and mutations. To investigate the expression patterns of genetic novelties across cell types, we performed single-cell RNA-sequencing of adult Drosophila testis. We found that new genes were expressed in various cell types, the patterns of which may be influenced by their mode of origination. In particular, lineage-specific de novo genes are commonly expressed in early spermatocytes, while young duplicated genes are often bimodally expressed. Analysis of germline substitutions suggests that spermatogenesis is a highly reparative process, with the mutational load of germ cells decreasing as spermatogenesis progresses. By elucidating the distribution of genetic novelties across spermatogenesis, this study provides a deeper understanding of how the testis maintains its core reproductive function while being a hotbed of evolutionary innovation.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted July 02, 2019.
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Testis single-cell RNA-seq reveals the dynamics of de novo gene transcription and germline mutational bias in Drosophila
Evan Witt, Sigi Benjamin, Nicolas Svetec, Li Zhao
bioRxiv 689687; doi: https://doi.org/10.1101/689687
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Testis single-cell RNA-seq reveals the dynamics of de novo gene transcription and germline mutational bias in Drosophila
Evan Witt, Sigi Benjamin, Nicolas Svetec, Li Zhao
bioRxiv 689687; doi: https://doi.org/10.1101/689687

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