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The potential for polyphosphate metabolism in Archaea and anaerobic polyphosphate formation in Methanosarcina mazei

Fabiana S. Paula, Jason Chin, Anna Schnurer, Bettina Muller, Panagiotis Manesiotis, Nicholas Waters, Katrina A. Macintosh, John P. Quinn, Jasmine Connolly, Florence Abram, John McGrath, Vincent O’Flaherty
doi: https://doi.org/10.1101/689885
Fabiana S. Paula
1Microbiology, School of Natural Sciences and Ryan Institute, National University of Ireland Galway, Galway, Republic of Ireland
2Department of Molecular Sciences, Biocenter, Swedish University of Agricultural Sciences, Uppsala, Sweden
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  • For correspondence: fabianaspaula@gmail.com vincent.oflaherty@nuigalway.ie
Jason Chin
3School of Biological Sciences and the Institute for Global Food Security, The Queen’s University of Belfast, Belfast, UK
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Anna Schnurer
2Department of Molecular Sciences, Biocenter, Swedish University of Agricultural Sciences, Uppsala, Sweden
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Bettina Muller
2Department of Molecular Sciences, Biocenter, Swedish University of Agricultural Sciences, Uppsala, Sweden
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Panagiotis Manesiotis
4School of Chemistry and Chemical Engineering, The Queen’s University of Belfast, Belfast, UK
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Nicholas Waters
1Microbiology, School of Natural Sciences and Ryan Institute, National University of Ireland Galway, Galway, Republic of Ireland
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Katrina A. Macintosh
3School of Biological Sciences and the Institute for Global Food Security, The Queen’s University of Belfast, Belfast, UK
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John P. Quinn
3School of Biological Sciences and the Institute for Global Food Security, The Queen’s University of Belfast, Belfast, UK
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Jasmine Connolly
1Microbiology, School of Natural Sciences and Ryan Institute, National University of Ireland Galway, Galway, Republic of Ireland
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Florence Abram
1Microbiology, School of Natural Sciences and Ryan Institute, National University of Ireland Galway, Galway, Republic of Ireland
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John McGrath
3School of Biological Sciences and the Institute for Global Food Security, The Queen’s University of Belfast, Belfast, UK
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Vincent O’Flaherty
1Microbiology, School of Natural Sciences and Ryan Institute, National University of Ireland Galway, Galway, Republic of Ireland
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  • For correspondence: fabianaspaula@gmail.com vincent.oflaherty@nuigalway.ie
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Abstract

Inorganic polyphosphate (polyP) is ubiquitous across all forms of life, but the study of its metabolism has been mainly confined to bacteria and yeasts. Few reports detail the presence and accumulation of polyP in Archaea, and little information is available on its functions and regulation. Here, we report that homologs of bacterial polyP metabolism proteins are present across the major taxa in the Archaea, suggesting that archaeal populations may have a greater contribution to global phosphorus cycling than has previously been recognised. We also demonstrate that polyP accumulation can be induced under strictly anaerobic conditions, in response to changes in phosphate (Pi) availability, i.e. Pi starvation, followed by incubation in Pi replete media (overplus), in cells of the methanogenic archaeon Methanosarcina mazei. Pi-starved M. mazei cells increased transcript abundance of the PHO-regulated alkaline phosphatase (phoA) gene and of the high-affinity phosphate transport (pstSCAB-phoU) operon: no increase in polyphosphate kinase 1 (ppk1) transcript abundance was observed. Subsequent incubation of Pi-starved M. mazei cells under Pi replete conditions, led to a 237% increase in intracellular polyphosphate content and a >5.7-fold increase in ppk1 gene transcripts. Ppk1 expression in M. mazei thus appears not to be under classical PHO regulon control.

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Posted July 02, 2019.
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The potential for polyphosphate metabolism in Archaea and anaerobic polyphosphate formation in Methanosarcina mazei
Fabiana S. Paula, Jason Chin, Anna Schnurer, Bettina Muller, Panagiotis Manesiotis, Nicholas Waters, Katrina A. Macintosh, John P. Quinn, Jasmine Connolly, Florence Abram, John McGrath, Vincent O’Flaherty
bioRxiv 689885; doi: https://doi.org/10.1101/689885
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The potential for polyphosphate metabolism in Archaea and anaerobic polyphosphate formation in Methanosarcina mazei
Fabiana S. Paula, Jason Chin, Anna Schnurer, Bettina Muller, Panagiotis Manesiotis, Nicholas Waters, Katrina A. Macintosh, John P. Quinn, Jasmine Connolly, Florence Abram, John McGrath, Vincent O’Flaherty
bioRxiv 689885; doi: https://doi.org/10.1101/689885

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