Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Genomic selection analyses reveal tradeoff between chestnut blight tolerance and genome inheritance from American chestnut (Castanea dentata) in (C. dentata x C. mollissima) x C. dentata backcross populations

Jared W. Westbrook, Qian Zhang, Mihir K. Mandal, Eric V. Jenkins, Laura E. Barth, Jerry W. Jenkins, Jane Grimwood, Jeremy Schmutz, Jason A. Holliday
doi: https://doi.org/10.1101/690693
Jared W. Westbrook
1The American Chestnut Foundation, Asheville, NC 28804
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: jared.westbrook@acf.org
Qian Zhang
2Virginia Tech, Department of Forest Resources and Environmental Conservation, Blacksburg, VA 24061
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mihir K. Mandal
3Claflin University, Department of Biology, Orangeburg, SC 29115
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eric V. Jenkins
1The American Chestnut Foundation, Asheville, NC 28804
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Laura E. Barth
1The American Chestnut Foundation, Asheville, NC 28804
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jerry W. Jenkins
4HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jane Grimwood
4HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jeremy Schmutz
4HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jason A. Holliday
2Virginia Tech, Department of Forest Resources and Environmental Conservation, Blacksburg, VA 24061
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

American chestnut was once a foundation species of eastern North American forests, but was rendered functionally extinct in the early 20th century by an exotic fungal blight (Cryphonectria parasitica). Over the past 30 years, The American Chestnut Foundation (TACF) has pursued backcross breeding to generate hybrids that combine the timber-type form of American chestnut with the blight tolerance of Chinese chestnut. The backcross strategy has been implemented based on the hypothesis that blight tolerance is conferred by few major effect alleles. We tested this hypothesis by developing genomic prediction models for five presence/absence blight phenotypes of 1,230 BC3F2 selection candidates and average canker severity of their BC3F3 progeny. We also genotyped pure Chinese and American chestnut reference panels to estimate the proportion of BC3F2 genomes inherited from parent species. We found that genomic prediction from a method that assumes an infinitesimal model of inheritance (HBLUP) has a similar predictive ability to a method that tends to perform well for traits controlled by major genes (Bayes C). Furthermore, the proportion of BC3F2 trees’ genomes inherited from American chestnut was negatively correlated with the blight tolerance of BC3F2 trees and their progeny. On average, selected BC3F2 trees inherited 83% of their genome from American chestnut and have blight-tolerance that is intermediate between F1 hybrids and American chestnut. Results suggest polygenic rather than major gene inheritance for blight tolerance. The blight-tolerance of restoration populations will be enhanced by advancing additional sources of blight-tolerance through fewer backcross generations and by potentially by breeding with transgenic blight-tolerant trees.

Footnotes

  • Email addresses (in author order): zqian{at}vt.edu, mimandal{at}claflin.edu, eric.jenkins{at}acf.org, laura.barth{at}acf.org, jjenkins{at}hudsonalpha.org, jgrimwood{at}hudsonalpha.org, jschmutz{at}hudsonalpha.org, jah1{at}vt.edu

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted July 03, 2019.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Genomic selection analyses reveal tradeoff between chestnut blight tolerance and genome inheritance from American chestnut (Castanea dentata) in (C. dentata x C. mollissima) x C. dentata backcross populations
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Genomic selection analyses reveal tradeoff between chestnut blight tolerance and genome inheritance from American chestnut (Castanea dentata) in (C. dentata x C. mollissima) x C. dentata backcross populations
Jared W. Westbrook, Qian Zhang, Mihir K. Mandal, Eric V. Jenkins, Laura E. Barth, Jerry W. Jenkins, Jane Grimwood, Jeremy Schmutz, Jason A. Holliday
bioRxiv 690693; doi: https://doi.org/10.1101/690693
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Genomic selection analyses reveal tradeoff between chestnut blight tolerance and genome inheritance from American chestnut (Castanea dentata) in (C. dentata x C. mollissima) x C. dentata backcross populations
Jared W. Westbrook, Qian Zhang, Mihir K. Mandal, Eric V. Jenkins, Laura E. Barth, Jerry W. Jenkins, Jane Grimwood, Jeremy Schmutz, Jason A. Holliday
bioRxiv 690693; doi: https://doi.org/10.1101/690693

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genomics
Subject Areas
All Articles
  • Animal Behavior and Cognition (3477)
  • Biochemistry (7315)
  • Bioengineering (5290)
  • Bioinformatics (20180)
  • Biophysics (9967)
  • Cancer Biology (7696)
  • Cell Biology (11242)
  • Clinical Trials (138)
  • Developmental Biology (6413)
  • Ecology (9910)
  • Epidemiology (2065)
  • Evolutionary Biology (13266)
  • Genetics (9346)
  • Genomics (12542)
  • Immunology (7665)
  • Microbiology (18919)
  • Molecular Biology (7413)
  • Neuroscience (40853)
  • Paleontology (298)
  • Pathology (1224)
  • Pharmacology and Toxicology (2124)
  • Physiology (3137)
  • Plant Biology (6833)
  • Scientific Communication and Education (1268)
  • Synthetic Biology (1890)
  • Systems Biology (5295)
  • Zoology (1083)