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Longitudinal impact of oral probiotic Lactobacillus acidophilus vaccine strains on the immune response and gut microbiome of mice

Zaid Abdo, Jonathan LeCureux, Alora LaVoy, Bridget Eklund, Elizabeth Ryan, Gregg Dean
doi: https://doi.org/10.1101/691709
Zaid Abdo
1Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA
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  • For correspondence: zaid.abdo@colostate.edu gregg.dean@colostate.edu
Jonathan LeCureux
1Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA
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Alora LaVoy
1Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA
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Bridget Eklund
1Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA
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Elizabeth Ryan
2Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, Colorado, USA
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Gregg Dean
1Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA
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  • For correspondence: zaid.abdo@colostate.edu gregg.dean@colostate.edu
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Abstract

The potential role of probiotic bacteria as adjuvants in vaccine trials led to their use as nonparenteral live mucosal vaccine vectors. Yet, interactions between these vectors, the host and the microbiome are poorly understood. This study evaluates impact of three probiotic, Lactobacillus acidophilus, vector strains, and their interactions with the host’s immune response, on the gut microbiome. One strain expressed the membrane proximal external region from HIV-1 (MPER). The other two expressed MPER and either secreted interleukin-1β (IL-1β) or expressed the surface flagellin subunit C (FliC) as adjuvants. We also used MPER with rice bran as prebiotic supplement. We observed a strain dependent, differential effect suggesting that MPER and IL-1β induced a shift of the microbiome while FliC had minimal impact. Joint probiotic and prebiotic use resulted in a compound effect, highlighting a potential synbiotic approach to impact efficacy of vaccination. Careful consideration of constitutive adjuvants and use of prebiotics is needed depending on whether or not to target microbiome modulation to improve vaccine efficacy. No clear associations were observed between total or MPER-specific IgA and the microbiome suggesting a role for other immune mechanisms or a need to focus on IgA-bound, resident microbiota, most affected by an immune response.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted July 03, 2019.
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Longitudinal impact of oral probiotic Lactobacillus acidophilus vaccine strains on the immune response and gut microbiome of mice
Zaid Abdo, Jonathan LeCureux, Alora LaVoy, Bridget Eklund, Elizabeth Ryan, Gregg Dean
bioRxiv 691709; doi: https://doi.org/10.1101/691709
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Longitudinal impact of oral probiotic Lactobacillus acidophilus vaccine strains on the immune response and gut microbiome of mice
Zaid Abdo, Jonathan LeCureux, Alora LaVoy, Bridget Eklund, Elizabeth Ryan, Gregg Dean
bioRxiv 691709; doi: https://doi.org/10.1101/691709

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