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Bordetella Colonization Factor A (BcfA) elicits protective immunity against Bordetella bronchiseptica in the absence of an additional adjuvant

Kacy S. Yount, Jamie Jennings-Gee, Kyle Caution, Audra R. Fullen, Kara N. Corps, Sally Quataert, Rajendar Deora, Purnima Dubey
doi: https://doi.org/10.1101/692830
Kacy S. Yount
1Department of Microbial Infection & Immunity, The Ohio State University
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Jamie Jennings-Gee
2Department of Microbiology & Immunology, Wake Forest University Health Sciences
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Kyle Caution
1Department of Microbial Infection & Immunity, The Ohio State University
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Audra R. Fullen
1Department of Microbial Infection & Immunity, The Ohio State University
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Kara N. Corps
3College of Veterinary Medicine, Department of Veterinary Biosciences, The Ohio State University
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Sally Quataert
4Human Immunology Center Laboratory, University of Rochester Medical Center
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Rajendar Deora
1Department of Microbial Infection & Immunity, The Ohio State University
5Department of Microbiology, The Ohio State University
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  • For correspondence: purnima.dubey@osumc.edu rajendar.deora@osumc.edu
Purnima Dubey
1Department of Microbial Infection & Immunity, The Ohio State University
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  • For correspondence: purnima.dubey@osumc.edu rajendar.deora@osumc.edu
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ABSTRACT

Bordetella bronchiseptica (B. bronchiseptica) is an etiologic agent of respiratory diseases in animals and humans. Despite widespread use of veterinary B. bronchiseptica vaccines, there is limited information on their composition, relative efficacy, and the immune responses they elicit. Furthermore, human B. bronchiseptica vaccines are not available. We leveraged the dual antigenic and adjuvant functions of BcfA to develop acellular B. bronchiseptica vaccines in the absence of an additional adjuvant. Balb/c mice immunized with BcfA alone or a trivalent vaccine containing BcfA and the Bordetella antigens FHA and Prn were equally protected against challenge with a prototype B. bronchiseptica strain. The trivalent vaccine protected mice significantly better than the canine vaccine Bronchicine® and provided protection against a B. bronchiseptica strain isolated from a dog with kennel cough. Th1/17-polarized immune responses correlate with long-lasting protection against Bordetellae and other respiratory pathogens. Notably, BcfA strongly attenuated the Th2 responses elicited by FHA/Prn, resulting in Th1/17-skewed responses in inherently Th2-skewed Balb/c mice. Thus, BcfA functions as both an antigen and an adjuvant, providing protection as a single component vaccine. BcfA-adjuvanted vaccines may improve the efficacy and durability of vaccines against Bordetellae and other pathogens.

Footnotes

  • Conflicts: RD holds a patent on BcfA (patent number US20150147332A1)

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted July 04, 2019.
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Bordetella Colonization Factor A (BcfA) elicits protective immunity against Bordetella bronchiseptica in the absence of an additional adjuvant
Kacy S. Yount, Jamie Jennings-Gee, Kyle Caution, Audra R. Fullen, Kara N. Corps, Sally Quataert, Rajendar Deora, Purnima Dubey
bioRxiv 692830; doi: https://doi.org/10.1101/692830
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Bordetella Colonization Factor A (BcfA) elicits protective immunity against Bordetella bronchiseptica in the absence of an additional adjuvant
Kacy S. Yount, Jamie Jennings-Gee, Kyle Caution, Audra R. Fullen, Kara N. Corps, Sally Quataert, Rajendar Deora, Purnima Dubey
bioRxiv 692830; doi: https://doi.org/10.1101/692830

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