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Domain-centric dissection and classification of prokaryotic poly(3-hydroxyalkanoate) synthases

Zhanzhong Liu, Zuobin Zhu, Jianye Yang, Sheng Wu, Qinghua Liu, View ORCID ProfileMengmeng Wang, Huiling Cheng, Jiawei Yan, Liang Wang
doi: https://doi.org/10.1101/693432
Zhanzhong Liu
1Xuzhou Infectious Diseases Hospital, Xuzhou Jiangsu, 221000, China
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Zuobin Zhu
2Department of Genetics, School of Biological Sciences and Technology, Xuzhou Medical University, Xuzhou Jiangsu, 221000, China
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Jianye Yang
3Department of Bioinformatics, School of Medical Informatics, Xuzhou Medical University, Xuzhou Jiangsu, 221000, China
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Sheng Wu
3Department of Bioinformatics, School of Medical Informatics, Xuzhou Medical University, Xuzhou Jiangsu, 221000, China
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Qinghua Liu
4Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, Xuzhou Jiangsu, 221000, China
5Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, Xuzhou Jiangsu, 221000, China
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Mengmeng Wang
4Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, Xuzhou Jiangsu, 221000, China
5Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, Xuzhou Jiangsu, 221000, China
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  • ORCID record for Mengmeng Wang
Huiling Cheng
3Department of Bioinformatics, School of Medical Informatics, Xuzhou Medical University, Xuzhou Jiangsu, 221000, China
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Jiawei Yan
1Xuzhou Infectious Diseases Hospital, Xuzhou Jiangsu, 221000, China
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Liang Wang
3Department of Bioinformatics, School of Medical Informatics, Xuzhou Medical University, Xuzhou Jiangsu, 221000, China
4Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, Xuzhou Jiangsu, 221000, China
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  • For correspondence: leonwang@xzhmu.edu.cn
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Abstract

Although many enzymes and multiple pathways involve in Polyhydroxyalkanoates (PHAs) synthesis, PHA synthases play a determinant role in the process, which include three subunits of PhaC, PhaE, and PhaR. Currently, PHA synthases are categorized into four classes according to its primary sequences, substrate specificity, and subunit composition. However, theoretical analysis of PHA synthases from the domain perspective has not been performed. In this study, we dissected PHA synthases thoroughly through analysis of domain organization. Both referenced bacterial and archaeal proteomes were then screened for the presence and absence of different PHA synthases along NCBI taxonomy ID-based phylogenetic tree. In addition, sequences annotated as bacterial and archaeal PhaCs in UniProt database were also analyzed for domain organizations and interactions. In sum, the in-silico study provided a better understanding of the domain features of PHA synthases in prokaryotes, which also assisted in the production of PHA polymers with optimized chemical properties.

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Posted July 05, 2019.
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Domain-centric dissection and classification of prokaryotic poly(3-hydroxyalkanoate) synthases
Zhanzhong Liu, Zuobin Zhu, Jianye Yang, Sheng Wu, Qinghua Liu, Mengmeng Wang, Huiling Cheng, Jiawei Yan, Liang Wang
bioRxiv 693432; doi: https://doi.org/10.1101/693432
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Domain-centric dissection and classification of prokaryotic poly(3-hydroxyalkanoate) synthases
Zhanzhong Liu, Zuobin Zhu, Jianye Yang, Sheng Wu, Qinghua Liu, Mengmeng Wang, Huiling Cheng, Jiawei Yan, Liang Wang
bioRxiv 693432; doi: https://doi.org/10.1101/693432

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