Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

An atlas of infiltrated B-lymphocytes in breast cancer revealed by paired single-cell RNA-sequencing and antigen receptor profiling

Qingtao Hu, Yu Hong, Pan Qi, Guangqing Lu, Xueying Mai, Sheng Xu, Xiaoying He, Yu Guo, Linlin Gao, Zhiyi Jing, Jiawen Wang, Tao Cai, Yu Zhang
doi: https://doi.org/10.1101/695601
Qingtao Hu
National Institute of Biological Sciences, Beijing 102206, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yu Hong
National Institute of Biological Sciences, Beijing 102206, China;Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, School of Life Sciences, Peking University, Beijing 100871, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pan Qi
Xinxiang central hospital, Xinxiang, Henan 453003, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Guangqing Lu
National Institute of Biological Sciences, Beijing 102206, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xueying Mai
National Institute of Biological Sciences, Beijing 102206, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sheng Xu
Xinxiang central hospital, Xinxiang, Henan 453003, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiaoying He
Xinxiang central hospital, Xinxiang, Henan 453003, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yu Guo
Xinxiang central hospital, Xinxiang, Henan 453003, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Linlin Gao
National Institute of Biological Sciences, Beijing 102206, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zhiyi Jing
National Institute of Biological Sciences, Beijing 102206, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jiawen Wang
National Institute of Biological Sciences, Beijing 102206, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tao Cai
National Institute of Biological Sciences, Beijing 102206, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yu Zhang
National Institute of Biological Sciences, Beijing 102206, China;Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, School of Life Sciences, Peking University, Beijing 100871, ChinaTsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing 100084, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: zhangyu@nibs.ac.cn
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

While it has been well-recognized that T-cell mediated adaptive cellular immunity plays important roles in cancer immune response and tumor control, the roles of B lymphocytes in tumor development and therapy have only been proposed until recently, and are still mostly controversial. To gain mechanistic insights into the origin and dynamics of tumor infiltrated immune cells, especially B lymphocytes, we combine single-cell RNA-sequencing and antigen receptor lineage analysis to characterize a large number of triple-negative breast cancer (TNBC) infiltrated immune cells and present a comprehensive atlas of infiltrated B-lymphocytes in TNBC, the most aggressive breast cancer subtype. We demonstrate that TNBC infiltrated B cells showed more mature and memory B cell characteristics, as well as high clonality and extensive IgH class switching recombination and somatic hypermutations. The B cell signatures based on single-cell RNA-seq results are significantly associated with improved survival for TNBC patients and provide better prognostication than classic single B cell markers (CD19 or CD20). Further dissection of the mechanisms regulating the functions and dynamic distribution of tumor infiltrated B cell populations will provide new clues for tumor immunotherapy.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted July 15, 2019.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
An atlas of infiltrated B-lymphocytes in breast cancer revealed by paired single-cell RNA-sequencing and antigen receptor profiling
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
Share
An atlas of infiltrated B-lymphocytes in breast cancer revealed by paired single-cell RNA-sequencing and antigen receptor profiling
Qingtao Hu, Yu Hong, Pan Qi, Guangqing Lu, Xueying Mai, Sheng Xu, Xiaoying He, Yu Guo, Linlin Gao, Zhiyi Jing, Jiawen Wang, Tao Cai, Yu Zhang
bioRxiv 695601; doi: https://doi.org/10.1101/695601
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
An atlas of infiltrated B-lymphocytes in breast cancer revealed by paired single-cell RNA-sequencing and antigen receptor profiling
Qingtao Hu, Yu Hong, Pan Qi, Guangqing Lu, Xueying Mai, Sheng Xu, Xiaoying He, Yu Guo, Linlin Gao, Zhiyi Jing, Jiawen Wang, Tao Cai, Yu Zhang
bioRxiv 695601; doi: https://doi.org/10.1101/695601

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Immunology
Subject Areas
All Articles
  • Animal Behavior and Cognition (1530)
  • Biochemistry (2488)
  • Bioengineering (1745)
  • Bioinformatics (9696)
  • Biophysics (3912)
  • Cancer Biology (2976)
  • Cell Biology (4210)
  • Clinical Trials (135)
  • Developmental Biology (2636)
  • Ecology (4107)
  • Epidemiology (2033)
  • Evolutionary Biology (6906)
  • Genetics (5217)
  • Genomics (6516)
  • Immunology (2191)
  • Microbiology (6967)
  • Molecular Biology (2763)
  • Neuroscience (17334)
  • Paleontology (126)
  • Pathology (430)
  • Pharmacology and Toxicology (708)
  • Physiology (1061)
  • Plant Biology (2496)
  • Scientific Communication and Education (646)
  • Synthetic Biology (832)
  • Systems Biology (2690)
  • Zoology (432)