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A unified role for membrane-cortex detachment during cell protrusion initiation

View ORCID ProfileErik S. Welf, Christopher E. Miles, Jaewon Huh, Meghan K. Driscoll, Tadamoto Isogai, Jungsik Noh, Andrew D. Weems, Joseph Chi, Theresa Pohlkamp, Kevin Dean, Reto Fiolka, Alex Mogilner, Gaudenz Danuser
doi: https://doi.org/10.1101/696211
Erik S. Welf
University of Texas Southwestern Medical Center, Dallas TX, USA
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  • ORCID record for Erik S. Welf
Christopher E. Miles
New York University, New York NY, USA
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Jaewon Huh
University of Texas Southwestern Medical Center, Dallas TX, USA
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Meghan K. Driscoll
University of Texas Southwestern Medical Center, Dallas TX, USA
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Tadamoto Isogai
University of Texas Southwestern Medical Center, Dallas TX, USA
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Jungsik Noh
University of Texas Southwestern Medical Center, Dallas TX, USA
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Andrew D. Weems
University of Texas Southwestern Medical Center, Dallas TX, USA
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Joseph Chi
University of Texas Southwestern Medical Center, Dallas TX, USA
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Theresa Pohlkamp
University of Texas Southwestern Medical Center, Dallas TX, USA
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Kevin Dean
University of Texas Southwestern Medical Center, Dallas TX, USA
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Reto Fiolka
University of Texas Southwestern Medical Center, Dallas TX, USA
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Alex Mogilner
New York University, New York NY, USA
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  • For correspondence: mogilner@cims.nyu.edu Gaudenz.Danuser@UTSouthwestern.edu
Gaudenz Danuser
University of Texas Southwestern Medical Center, Dallas TX, USA
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  • For correspondence: mogilner@cims.nyu.edu Gaudenz.Danuser@UTSouthwestern.edu
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Abstract

Cell morphogenesis employs a diversity of membrane protrusions. They are discriminated by differences in force generation. Actin polymerization is the best studied mechanism of force generation, but growing interest in how variable molecular conditions and microenvironments alter morphogenesis has revealed other mechanisms, including intracellular pressure. Here, we show that local depletion of membrane cortex links is an essential step in the initiation of both pressure-based and actin-based protrusions. This observation challenges the quarter-century old Brownian ratchet model of actin-driven membrane protrusion, which requires an optimal balance of actin filament growth and membrane tethering. An updated model confirms membrane-filament detachment is necessary to activate the ratchet mechanism. These findings unify the regulation of different protrusion types, explaining how cells generate robust yet flexible strategies of morphogenesis.

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Posted July 08, 2019.
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A unified role for membrane-cortex detachment during cell protrusion initiation
Erik S. Welf, Christopher E. Miles, Jaewon Huh, Meghan K. Driscoll, Tadamoto Isogai, Jungsik Noh, Andrew D. Weems, Joseph Chi, Theresa Pohlkamp, Kevin Dean, Reto Fiolka, Alex Mogilner, Gaudenz Danuser
bioRxiv 696211; doi: https://doi.org/10.1101/696211
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A unified role for membrane-cortex detachment during cell protrusion initiation
Erik S. Welf, Christopher E. Miles, Jaewon Huh, Meghan K. Driscoll, Tadamoto Isogai, Jungsik Noh, Andrew D. Weems, Joseph Chi, Theresa Pohlkamp, Kevin Dean, Reto Fiolka, Alex Mogilner, Gaudenz Danuser
bioRxiv 696211; doi: https://doi.org/10.1101/696211

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