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Tissue mechanics drives epithelialization, goblet cell regeneration, and restoration of a mucociliated epidermis on the surface of embryonic aggregates

Hye Young Kim, Timothy R. Jackson, Carsten Stuckenholz, View ORCID ProfileLance A. Davidson
doi: https://doi.org/10.1101/696997
Hye Young Kim
1Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh PA 15213, U.S.A.
4Institute for Basic Science, Daejeon, Republic of Korea
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  • For correspondence: lad43@pitt.edu hyeyoung.pitt@gmail.com
Timothy R. Jackson
1Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh PA 15213, U.S.A.
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Carsten Stuckenholz
1Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh PA 15213, U.S.A.
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Lance A. Davidson
1Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh PA 15213, U.S.A.
2Department of Developmental Biology, School of Medicine, University of Pittsburgh, Pittsburgh PA 15260, U.S.A.
3Department of Computational and Systems Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15260, U.S.A.
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  • ORCID record for Lance A. Davidson
  • For correspondence: lad43@pitt.edu hyeyoung.pitt@gmail.com
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Abstract

Injury, surgery, and disease often disrupt tissues and it is the process of regeneration that aids the restoration of architecture and function. Regeneration can occur through multiple strategies including induction of stem cell expansion, transdifferentiation, or proliferation of differentiated cells. We have uncovered a case of regeneration that restores a mucociliated epithelium from mesenchymal cells. Following disruption of embryonic tissue architecture and assembly of a compact mesenchymal aggregate, regeneration first involves restoration of an epithelium, transitioning from mesenchymal cells at the surface of the aggregate. Cells establish apico-basal polarity within 5 hours and a mucociliated epithelium within 24. Regeneration coincides with nuclear translocation of the putative mechanotransducer YAP1 and a sharp increase in aggregate stiffness, and regeneration can be controlled by altering stiffness. We propose that regeneration of a mucociliated epithelium occurs in response to biophysical cues sensed by newly exposed cells on the surface of a disrupted mesenchymal tissue.

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Posted July 12, 2019.
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Tissue mechanics drives epithelialization, goblet cell regeneration, and restoration of a mucociliated epidermis on the surface of embryonic aggregates
Hye Young Kim, Timothy R. Jackson, Carsten Stuckenholz, Lance A. Davidson
bioRxiv 696997; doi: https://doi.org/10.1101/696997
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Tissue mechanics drives epithelialization, goblet cell regeneration, and restoration of a mucociliated epidermis on the surface of embryonic aggregates
Hye Young Kim, Timothy R. Jackson, Carsten Stuckenholz, Lance A. Davidson
bioRxiv 696997; doi: https://doi.org/10.1101/696997

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