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Microbiota supplementation with Bifidobacterium and Lactobacillus modifies the preterm infant gut microbiota and metabolome

Cristina Alcon-Giner, Matthew J. Dalby, Shabhonam Caim, Jennifer Ketskemety, Alex Shaw, Kathleen Sim, Melissa Lawson, Raymond Kiu, Charlotte Leclaire, Lisa Chalklen, Magdalena Kujawska, Suparna Mitra, Fahmina Fardus-Reid, Gustav Belteki, Katherine Lloyd, Jonathan R. Swann, J. Simon Kroll, Paul Clarke, View ORCID ProfileLindsay J. Hall
doi: https://doi.org/10.1101/698092
Cristina Alcon-Giner
1Quadram Institute Bioscience, Norwich Research Park, Norwich, UK
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Matthew J. Dalby
1Quadram Institute Bioscience, Norwich Research Park, Norwich, UK
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Shabhonam Caim
1Quadram Institute Bioscience, Norwich Research Park, Norwich, UK
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Jennifer Ketskemety
1Quadram Institute Bioscience, Norwich Research Park, Norwich, UK
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Alex Shaw
2Department of Medicine, Section of Pediatrics, Imperial College London, London, UK
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Kathleen Sim
2Department of Medicine, Section of Pediatrics, Imperial College London, London, UK
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Melissa Lawson
1Quadram Institute Bioscience, Norwich Research Park, Norwich, UK
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Raymond Kiu
1Quadram Institute Bioscience, Norwich Research Park, Norwich, UK
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Charlotte Leclaire
1Quadram Institute Bioscience, Norwich Research Park, Norwich, UK
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Lisa Chalklen
1Quadram Institute Bioscience, Norwich Research Park, Norwich, UK
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Magdalena Kujawska
1Quadram Institute Bioscience, Norwich Research Park, Norwich, UK
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Suparna Mitra
1Quadram Institute Bioscience, Norwich Research Park, Norwich, UK
3Leeds Institute of Medical Research, University of Leeds, Leeds, UK
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Fahmina Fardus-Reid
4Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, UK
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Gustav Belteki
5Neonatal Intensive Care Unit, The Rosie Hospital, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK
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Katherine Lloyd
6Neonatal Intensive Care Unit, Norfolk and Norwich University Hospital, Norwich, UK
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Jonathan R. Swann
4Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, UK
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J. Simon Kroll
2Department of Medicine, Section of Pediatrics, Imperial College London, London, UK
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Paul Clarke
6Neonatal Intensive Care Unit, Norfolk and Norwich University Hospital, Norwich, UK
7Norwich Medical School, University of East Anglia, Norwich, UK
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Lindsay J. Hall
1Quadram Institute Bioscience, Norwich Research Park, Norwich, UK
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  • ORCID record for Lindsay J. Hall
  • For correspondence: lindsay.hall@quadram.ac.uk
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Abstract

Supplementation with members of the early-life microbiota or ‘probiotics’ is becoming increasingly popular to attempt to beneficially manipulate the preterm gut microbiota. We performed a large longitudinal study comprising two preterm groups; 101 orally supplemented with Bifidobacterium and Lactobacillus (Bif/Lacto) and 133 non-supplemented (Control) matched by age, sex, birth-mode, and diet. 16S rRNA metataxonomic profiling on stool samples (n = 592) indicated a predominance of Bifidobacterium, and a reduction of pathobionts in the Bif/Lacto group. Metabolic phenotyping found a parallel increase in fecal acetate and lactate in the Bif/Lacto group compared to the Control group, which positively correlated with Bifidobacterium abundance consistent with the ability of the supplemented Bifidobacterium strain to metabolize human milk oligosaccharides and reduced gut pH. This study demonstrates that microbiota supplementation can modify the preterm microbiome and the gastrointestinal environment to more closely resemble that of a full-term infant.

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Posted July 11, 2019.
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Microbiota supplementation with Bifidobacterium and Lactobacillus modifies the preterm infant gut microbiota and metabolome
Cristina Alcon-Giner, Matthew J. Dalby, Shabhonam Caim, Jennifer Ketskemety, Alex Shaw, Kathleen Sim, Melissa Lawson, Raymond Kiu, Charlotte Leclaire, Lisa Chalklen, Magdalena Kujawska, Suparna Mitra, Fahmina Fardus-Reid, Gustav Belteki, Katherine Lloyd, Jonathan R. Swann, J. Simon Kroll, Paul Clarke, Lindsay J. Hall
bioRxiv 698092; doi: https://doi.org/10.1101/698092
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Microbiota supplementation with Bifidobacterium and Lactobacillus modifies the preterm infant gut microbiota and metabolome
Cristina Alcon-Giner, Matthew J. Dalby, Shabhonam Caim, Jennifer Ketskemety, Alex Shaw, Kathleen Sim, Melissa Lawson, Raymond Kiu, Charlotte Leclaire, Lisa Chalklen, Magdalena Kujawska, Suparna Mitra, Fahmina Fardus-Reid, Gustav Belteki, Katherine Lloyd, Jonathan R. Swann, J. Simon Kroll, Paul Clarke, Lindsay J. Hall
bioRxiv 698092; doi: https://doi.org/10.1101/698092

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