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Artifact Formation in Single Molecule Localization Microscopy

Jochen M. Reichel, Thomas Vomhof, View ORCID ProfileJens Michaelis
doi: https://doi.org/10.1101/700955
Jochen M. Reichel
1Institute for Biophysics, Ulm University, Ulm, Germany
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Thomas Vomhof
1Institute for Biophysics, Ulm University, Ulm, Germany
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Jens Michaelis
1Institute for Biophysics, Ulm University, Ulm, Germany
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  • For correspondence: jens.michaelis@uni-ulm.de
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Abstract

We investigate the influence of different accuracy-detection rate trade-offs on image reconstruction in single molecule localization microscopy. Our main focus is the investigation of image artifacts experienced when using low localization accuracy, especially in the presence of sample drift and inhomogeneous background. In this context we present a newly developed SMLM software termed FIRESTORM which is optimized for high accuracy reconstruction. For our analysis we used in silico SMLM data and compared the reconstructed images to the ground truth data. We observe two discriminable reconstruction populations of which only one shows the desired localization behavior.

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Posted July 12, 2019.
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Artifact Formation in Single Molecule Localization Microscopy
Jochen M. Reichel, Thomas Vomhof, Jens Michaelis
bioRxiv 700955; doi: https://doi.org/10.1101/700955
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Artifact Formation in Single Molecule Localization Microscopy
Jochen M. Reichel, Thomas Vomhof, Jens Michaelis
bioRxiv 700955; doi: https://doi.org/10.1101/700955

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