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Estradiol-inducible AvrRps4 expression reveals distinct properties of TIR-NLR-mediated effector-triggered immunity

View ORCID ProfileBruno Pok Man Ngou, View ORCID ProfileHee-Kyung Ahn, View ORCID ProfilePingtao Ding, View ORCID ProfileAmey Redkar, Hannah Brown, Yan Ma, Mark Youles, View ORCID ProfileLaurence Tomlinson, View ORCID ProfileJonathan DG Jones
doi: https://doi.org/10.1101/701359
Bruno Pok Man Ngou
1The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK
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Hee-Kyung Ahn
1The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK
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Pingtao Ding
1The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK
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  • For correspondence: pingtao.ding@tsl.ac.uk jonathan.jones@tsl.ac.uk
Amey Redkar
1The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK
3Department of Genetics, University of Córdoba, Córdoba 14071, Spain
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Hannah Brown
1The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK
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Yan Ma
1The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK
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Mark Youles
1The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK
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Laurence Tomlinson
1The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK
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Jonathan DG Jones
1The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK
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  • ORCID record for Jonathan DG Jones
  • For correspondence: pingtao.ding@tsl.ac.uk jonathan.jones@tsl.ac.uk
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Abstract

Plant nucleotide-binding domain, leucine-rich repeat receptor (NLR) proteins play important roles in recognition of pathogen-derived effectors. However, the mechanism by which plant NLRs activate immunity is still largely unknown. The paired Arabidopsis NLRs RRS1-R and RPS4, that confer recognition of bacterial effectors AvrRps4 and PopP2, are well studied, but how the RRS1/RPS4 complex activates early immediate downstream responses upon effector detection is still poorly understood. To study RRS1/RPS4 responses without the influence of cell-surface receptor immune pathways, we generated an Arabidopsis line with inducible expression of effector AvrRps4. Induction does not lead to hypersensitive cell death response (HR) but can induce electrolyte leakage, which often correlates with plant cell death. Activation of RRS1 and RPS4 without pathogens cannot activate mitogen-associated protein kinase cascades, but still activates upregulation of defense genes, and therefore resistance against bacteria.

Highlight Inducible expression of AvrRps4 activates RRS1/RPS4-mediated effector-triggered immunity without the presence of pathogens, allowing us to characterise downstream immune responses triggered by TIR-NLRs without cell-surface receptor-mediated immunity.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted July 14, 2019.
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Estradiol-inducible AvrRps4 expression reveals distinct properties of TIR-NLR-mediated effector-triggered immunity
Bruno Pok Man Ngou, Hee-Kyung Ahn, Pingtao Ding, Amey Redkar, Hannah Brown, Yan Ma, Mark Youles, Laurence Tomlinson, Jonathan DG Jones
bioRxiv 701359; doi: https://doi.org/10.1101/701359
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Estradiol-inducible AvrRps4 expression reveals distinct properties of TIR-NLR-mediated effector-triggered immunity
Bruno Pok Man Ngou, Hee-Kyung Ahn, Pingtao Ding, Amey Redkar, Hannah Brown, Yan Ma, Mark Youles, Laurence Tomlinson, Jonathan DG Jones
bioRxiv 701359; doi: https://doi.org/10.1101/701359

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