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GenEPi: Piezo1-based fluorescent reporter for visualizing mechanical stimuli with high spatiotemporal resolution

Sine Yaganoglu, Nordine Helassa, Benjamin M. Gaub, Maaike Welling, Jian Shi, Daniel J. Müller, Katalin Török, View ORCID ProfilePeriklis Pantazis
doi: https://doi.org/10.1101/702423
Sine Yaganoglu
1Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich, 4058 Basel, Switzerland
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Nordine Helassa
2Molecular and Clinical Sciences Research Institute, St. George’s, University of London, UK
3Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, UK
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Benjamin M. Gaub
1Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich, 4058 Basel, Switzerland
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Maaike Welling
1Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich, 4058 Basel, Switzerland
4Department of Bioengineering, Imperial College London, South Kensington Campus, London SW7 2AZ, UK
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Jian Shi
5Leeds Institute of Cardiovascular and Metabolic Medicine, LIGHT Laboratories, University of Leeds, Leeds, LS2 9JT, UK
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Daniel J. Müller
1Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich, 4058 Basel, Switzerland
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Katalin Török
2Molecular and Clinical Sciences Research Institute, St. George’s, University of London, UK
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Periklis Pantazis
1Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich, 4058 Basel, Switzerland
4Department of Bioengineering, Imperial College London, South Kensington Campus, London SW7 2AZ, UK
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  • ORCID record for Periklis Pantazis
  • For correspondence: p.pantazis@imperial.ac.uk
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Abstract

Mechanosensing is a ubiquitous process to translate external mechanical stimuli into biological responses during development, homeostasis, and disease. However, non-invasive investigation of cellular mechanosensing in complex and intact live tissue remains challenging. Here, we developed GenEPi, a genetically-encoded fluorescent intensiometric reporter for mechanical stimuli based on Piezo1, an essential mechanosensitive ion channel found in vertebrates. We show that GenEPi has high specificity and spatiotemporal resolution for Piezo1-dependent mechanical stimuli, exemplified by resolving repetitive mechanical stimuli of spontaneously contracting cardiomyocytes within microtissues, in a non-invasive manner.

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Posted July 15, 2019.
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GenEPi: Piezo1-based fluorescent reporter for visualizing mechanical stimuli with high spatiotemporal resolution
Sine Yaganoglu, Nordine Helassa, Benjamin M. Gaub, Maaike Welling, Jian Shi, Daniel J. Müller, Katalin Török, Periklis Pantazis
bioRxiv 702423; doi: https://doi.org/10.1101/702423
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GenEPi: Piezo1-based fluorescent reporter for visualizing mechanical stimuli with high spatiotemporal resolution
Sine Yaganoglu, Nordine Helassa, Benjamin M. Gaub, Maaike Welling, Jian Shi, Daniel J. Müller, Katalin Török, Periklis Pantazis
bioRxiv 702423; doi: https://doi.org/10.1101/702423

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