Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

A role for the P2Y1 receptor in nonsynaptic cross-depolarization in the rat dorsal root ganglia

Gil B. Carvalho, Yatendra Mulpuri, Antonio Damasio, Igor Spigelman
doi: https://doi.org/10.1101/702563
Gil B. Carvalho
Brain and Creativity Institute, University of Southern California, 3620 A McClintock Avenue, Suite 265, Los Angeles, California 90089-2921, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yatendra Mulpuri
Division of Oral Biology & Medicine, School of Dentistry, University of California, 10833 Le Conte Avenue, 63-078 CHS, Los Angeles, California 090095-1668, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Antonio Damasio
Brain and Creativity Institute, University of Southern California, 3620 A McClintock Avenue, Suite 265, Los Angeles, California 90089-2921, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: igor@ucla.edu damasio@usc.edu
Igor Spigelman
Division of Oral Biology & Medicine, School of Dentistry, University of California, 10833 Le Conte Avenue, 63-078 CHS, Los Angeles, California 090095-1668, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: igor@ucla.edu damasio@usc.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Non-synaptic transmission is pervasive throughout the nervous system. It appears especially prevalent in peripheral ganglia, where non-synaptic interactions between neighboring cell bodies have been described in both physiological and pathological conditions, a phenomenon referred to as cross-depolarization (CD) and thought to play a role in sensory processing and chronic pain. CD has been proposed to be mediated by a chemical agent, but its identity has remained elusive. Here, we report that in the rat dorsal root ganglion (DRG), the P2Y1 purinergic receptor (P2RY1) plays an important role in regulating CD. The effect of P2RY1 is cell-type specific: pharmacological blockade of P2RY1 inhibited CD in A-type neurons while enhancing it in unmyelinated C-type cells. In the nodose ganglion of the vagus, CD requires extracellular calcium in a large percentage of cells. In contrast, we show that in the DRG extracellular calcium appears to play no major role, pointing to a mechanistic difference between the two peripheral ganglia. Furthermore, we show that DRG glial cells also play a cell-type specific role in CD regulation. Fluorocitrate-induced glial inactivation had no effect on A-cells but enhanced CD in C-cells. These findings shed light on the mechanism of CD in the DRG and pave the way for further analysis of non-synaptic neuronal communication in sensory ganglia.

Highlights

  • The purinergic receptor P2RY1 plays a regulatory role in non-synaptic crossdepolarization (CD) in the mammalian DRG

  • The effect of P2RY1 is cell type-specific: it enhances CD in myelinated A-type neurons, but inhibits it in unmyelinated C-cells

  • CD in the DRG does not require extracellular calcium. This is in contrast with the nodose ganglion, where extracellular calcium plays an important role in nonsynaptic interactions

  • CD is also modulated by DRG glial cells. Glia selectively inhibit CD in C-type neurons

Footnotes

  • The authors declare no conflict of interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted July 14, 2019.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
A role for the P2Y1 receptor in nonsynaptic cross-depolarization in the rat dorsal root ganglia
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
Share
A role for the P2Y1 receptor in nonsynaptic cross-depolarization in the rat dorsal root ganglia
Gil B. Carvalho, Yatendra Mulpuri, Antonio Damasio, Igor Spigelman
bioRxiv 702563; doi: https://doi.org/10.1101/702563
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
A role for the P2Y1 receptor in nonsynaptic cross-depolarization in the rat dorsal root ganglia
Gil B. Carvalho, Yatendra Mulpuri, Antonio Damasio, Igor Spigelman
bioRxiv 702563; doi: https://doi.org/10.1101/702563

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (1525)
  • Biochemistry (2480)
  • Bioengineering (1737)
  • Bioinformatics (9676)
  • Biophysics (3900)
  • Cancer Biology (2971)
  • Cell Biology (4194)
  • Clinical Trials (135)
  • Developmental Biology (2627)
  • Ecology (4102)
  • Epidemiology (2031)
  • Evolutionary Biology (6898)
  • Genetics (5206)
  • Genomics (6500)
  • Immunology (2184)
  • Microbiology (6944)
  • Molecular Biology (2752)
  • Neuroscience (17280)
  • Paleontology (126)
  • Pathology (427)
  • Pharmacology and Toxicology (706)
  • Physiology (1056)
  • Plant Biology (2489)
  • Scientific Communication and Education (643)
  • Synthetic Biology (831)
  • Systems Biology (2689)
  • Zoology (430)