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Identification of a major QTL and associated marker for high arabinoxylan fibre in white wheat flour

Alison Lovegrove, Luzie U. Wingen, Amy Plummer, Abigail Wood, Diana Passmore, Ondrej Kosik, Jackie Freeman, Rowan A.C. Mitchell, Mehmet Ulker, Karolina Tremmel-Bede, Marianna Rakszegi, Zoltán Bedő, Marie-Reine Petterant, Gilles Charmet, Michelle Leverington Waite, Simon Orford, Amanda Burridge, Till Pellny, Peter R Shewry, Simon Griffiths
doi: https://doi.org/10.1101/705343
Alison Lovegrove
1Rothamsted Research, West Common, Herts AL5 2JQ
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Luzie U. Wingen
2John Innes Centre, Norwich Research Park, Colney Lane, Norwich NR4 7UH
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Amy Plummer
1Rothamsted Research, West Common, Herts AL5 2JQ
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Abigail Wood
1Rothamsted Research, West Common, Herts AL5 2JQ
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Diana Passmore
1Rothamsted Research, West Common, Herts AL5 2JQ
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Ondrej Kosik
1Rothamsted Research, West Common, Herts AL5 2JQ
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Jackie Freeman
1Rothamsted Research, West Common, Herts AL5 2JQ
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Rowan A.C. Mitchell
1Rothamsted Research, West Common, Herts AL5 2JQ
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Mehmet Ulker
3Bardakçi Mahallesi, Yüzüncü Yil Üniversitesi Kampüsü, 65090 Tuşba/Van, Turkey
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Karolina Tremmel-Bede
4Centre for Agricultural Research, Hungarian Academy of Sceinces, Martonvásár, Hungary
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Marianna Rakszegi
4Centre for Agricultural Research, Hungarian Academy of Sceinces, Martonvásár, Hungary
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Zoltán Bedő
4Centre for Agricultural Research, Hungarian Academy of Sceinces, Martonvásár, Hungary
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Marie-Reine Petterant
5INRA, 5 Chemin de Beaulieu, 63000 Clermont-Ferrand, France
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Gilles Charmet
5INRA, 5 Chemin de Beaulieu, 63000 Clermont-Ferrand, France
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Michelle Leverington Waite
2John Innes Centre, Norwich Research Park, Colney Lane, Norwich NR4 7UH
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Simon Orford
2John Innes Centre, Norwich Research Park, Colney Lane, Norwich NR4 7UH
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Amanda Burridge
6Life Sciences, University of Bristol, 24 Tyndall Avenue, Bristol, BS8 1TQ
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Till Pellny
1Rothamsted Research, West Common, Herts AL5 2JQ
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Peter R Shewry
1Rothamsted Research, West Common, Herts AL5 2JQ
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  • For correspondence: peter.shewry@rothamsted.ac.uk
Simon Griffiths
5INRA, 5 Chemin de Beaulieu, 63000 Clermont-Ferrand, France
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Abstract

Dietary fibre (DF) has multiple health benefits, and wheat products are major sources of DF for human health. However, DF is depleted in white flour, which is most widely consumed, compared to wholegrain. The major type of DF in white wheat flour is the cell wall polysaccharide arabinoxylan (AX). Previous studies have identified the Chinese wheat cultivar Yumai 34 as having unusually high contents of AX in both water-soluble and insoluble forms. We have therefore used populations generated from crosses between Yumai 34 and four other wheat cultivars, three with average contents of AX (Ukrainka, Altigo and Claire) and one also having unusually high AX (Valoris), in order to map QTLs for soluble AX (determined as relative viscosity) of aqueous extracts of wholemeal flours) and total AX (determined by enzyme fingerprinting of white flour). A number of QTL were mapped, but most were only detected in one or two crosses. However, all four crosses showed strong QTLs for high RV/total AX on chromosome 1B, with Yumai 34 being the increasing parent, and a KASP marker for the high AX Yumai 34 allele was validated by analysis of high AX lines derived from Yumai 34 but selected by biochemical analysis. A QTL for RV was mapped on chromosome 6B in Yumai 34 × Valoris, with Valoris being the increasing allele, which was consistent with the observation of transgressive segregation for this trait. The data indicate that breeding can be used to develop wheat with high AX fibre in white flour.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted July 17, 2019.
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Identification of a major QTL and associated marker for high arabinoxylan fibre in white wheat flour
Alison Lovegrove, Luzie U. Wingen, Amy Plummer, Abigail Wood, Diana Passmore, Ondrej Kosik, Jackie Freeman, Rowan A.C. Mitchell, Mehmet Ulker, Karolina Tremmel-Bede, Marianna Rakszegi, Zoltán Bedő, Marie-Reine Petterant, Gilles Charmet, Michelle Leverington Waite, Simon Orford, Amanda Burridge, Till Pellny, Peter R Shewry, Simon Griffiths
bioRxiv 705343; doi: https://doi.org/10.1101/705343
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Identification of a major QTL and associated marker for high arabinoxylan fibre in white wheat flour
Alison Lovegrove, Luzie U. Wingen, Amy Plummer, Abigail Wood, Diana Passmore, Ondrej Kosik, Jackie Freeman, Rowan A.C. Mitchell, Mehmet Ulker, Karolina Tremmel-Bede, Marianna Rakszegi, Zoltán Bedő, Marie-Reine Petterant, Gilles Charmet, Michelle Leverington Waite, Simon Orford, Amanda Burridge, Till Pellny, Peter R Shewry, Simon Griffiths
bioRxiv 705343; doi: https://doi.org/10.1101/705343

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