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Microglia are necessary for normal functional development of adult-born neurons in the olfactory bulb

View ORCID ProfileJenelle Wallace, Julia Lord, Lasse Dissing-Olesen, Beth Stevens, View ORCID ProfileVenkatesh Murthy
doi: https://doi.org/10.1101/714337
Jenelle Wallace
1Molecules, Cells, and Organisms training program
2Center for Brain Science, Harvard University, Cambridge, MA 02138, USA
3Department of Molecular & Cellular Biology, Harvard University, Cambridge, MA 02138, USA
4Boston Children’s Hospital, F.M. Kirby Neurobiology Center, Boston, MA, USA
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  • ORCID record for Jenelle Wallace
Julia Lord
3Department of Molecular & Cellular Biology, Harvard University, Cambridge, MA 02138, USA
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Lasse Dissing-Olesen
4Boston Children’s Hospital, F.M. Kirby Neurobiology Center, Boston, MA, USA
5Harvard Medical School, Boston, MA, USA
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Beth Stevens
4Boston Children’s Hospital, F.M. Kirby Neurobiology Center, Boston, MA, USA
5Harvard Medical School, Boston, MA, USA
6Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, USA
7Howard Hughes Medical Institute, Boston Children’s Hospital, Boston, MA, USA
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  • For correspondence: beth.stevens@childrens.harvard.edu vnmurthy@fas.harvard.edu
Venkatesh Murthy
1Molecules, Cells, and Organisms training program
2Center for Brain Science, Harvard University, Cambridge, MA 02138, USA
3Department of Molecular & Cellular Biology, Harvard University, Cambridge, MA 02138, USA
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  • ORCID record for Venkatesh Murthy
  • For correspondence: beth.stevens@childrens.harvard.edu vnmurthy@fas.harvard.edu
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Abstract

Microglia play key roles in regulating synapse development and refinement in the developing brain, but it is unknown whether they are similarly involved during adult neurogenesis. By transiently ablating microglia from the healthy adult mouse brain, we show that microglia are necessary for the normal functional development of adult-born granule cells (abGCs) in the olfactory bulb. Microglia ablation reduces the odor responses of developing, but not preexisting GCs in vivo in both awake and anesthetized mice. Microglia preferentially target their motile processes to interact with mushroom spines on abGCs, and when microglia are absent, abGCs develop smaller spines and receive weaker excitatory synaptic inputs. These results suggest that microglia promote the development of excitatory synapses onto developing abGCs, which may impact the function of these cells in the olfactory circuit.

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Posted July 25, 2019.
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Microglia are necessary for normal functional development of adult-born neurons in the olfactory bulb
Jenelle Wallace, Julia Lord, Lasse Dissing-Olesen, Beth Stevens, Venkatesh Murthy
bioRxiv 714337; doi: https://doi.org/10.1101/714337
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Microglia are necessary for normal functional development of adult-born neurons in the olfactory bulb
Jenelle Wallace, Julia Lord, Lasse Dissing-Olesen, Beth Stevens, Venkatesh Murthy
bioRxiv 714337; doi: https://doi.org/10.1101/714337

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