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Titrating gene expression with series of systematically compromised CRISPR guide RNAs

View ORCID ProfileMarco Jost, Daniel A. Santos, Reuben A. Saunders, Max A. Horlbeck, John S. Hawkins, Sonia M. Scaria, Thomas M. Norman, Jeffrey A. Hussmann, Christina R. Liem, Carol A. Gross, Jonathan S. Weissman
doi: https://doi.org/10.1101/717389
Marco Jost
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USAHoward Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USACalifornia Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, CA 94158, USADepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA
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  • ORCID record for Marco Jost
Daniel A. Santos
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USAHoward Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USACalifornia Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, CA 94158, USA
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Reuben A. Saunders
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USAHoward Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USACalifornia Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, CA 94158, USA
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Max A. Horlbeck
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USAHoward Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USACalifornia Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, CA 94158, USA
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John S. Hawkins
Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA
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Sonia M. Scaria
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USAHoward Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USACalifornia Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, CA 94158, USA
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Thomas M. Norman
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USAHoward Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USACalifornia Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, CA 94158, USA
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Jeffrey A. Hussmann
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USAHoward Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USACalifornia Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, CA 94158, USADepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA
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Christina R. Liem
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USAHoward Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USACalifornia Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, CA 94158, USA
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Carol A. Gross
Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USADepartment of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA, USA
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Jonathan S. Weissman
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USAHoward Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USACalifornia Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, CA 94158, USA
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  • For correspondence: jonathan.weissman@ucsf.edu
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Article Information

doi 
https://doi.org/10.1101/717389
History 
  • July 28, 2019.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.

Author Information

  1. Marco Jost1,2,3,4,*,
  2. Daniel A. Santos1,2,3,*,
  3. Reuben A. Saunders1,2,3,
  4. Max A. Horlbeck1,2,3,
  5. John S. Hawkins4,
  6. Sonia M. Scaria1,2,3,
  7. Thomas M. Norman1,2,3,
  8. Jeffrey A. Hussmann1,2,3,4,
  9. Christina R. Liem1,2,3,
  10. Carol A. Gross4,5 and
  11. Jonathan S. Weissman1,2,3,#
  1. 1Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA
  2. 2Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA
  3. 3California Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, CA 94158, USA
  4. 4Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA
  5. 5Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA, USA
  1. ↵#Correspondence should be addressed to: jonathan.weissman{at}ucsf.edu
  1. ↵* These authors contributed equally

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Titrating gene expression with series of systematically compromised CRISPR guide RNAs
Marco Jost, Daniel A. Santos, Reuben A. Saunders, Max A. Horlbeck, John S. Hawkins, Sonia M. Scaria, Thomas M. Norman, Jeffrey A. Hussmann, Christina R. Liem, Carol A. Gross, Jonathan S. Weissman
bioRxiv 717389; doi: https://doi.org/10.1101/717389
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Titrating gene expression with series of systematically compromised CRISPR guide RNAs
Marco Jost, Daniel A. Santos, Reuben A. Saunders, Max A. Horlbeck, John S. Hawkins, Sonia M. Scaria, Thomas M. Norman, Jeffrey A. Hussmann, Christina R. Liem, Carol A. Gross, Jonathan S. Weissman
bioRxiv 717389; doi: https://doi.org/10.1101/717389

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