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Native and synthetic methanol assimilation in Saccharomyces cerevisiae

Monica I. Espinosa, Kaspar Valgepea, Ricardo A. Gonzalez-Garcia, Colin Scott, Isak S. Pretorius, Esteban Marcellin, Ian T. Paulsen, Thomas C. Williams
doi: https://doi.org/10.1101/717942
Monica I. Espinosa
1Department of Molecular Sciences, Macquarie University, Sydney, Australia
2Synthetic Biology Future Science Platform, CSIRO, Sydney, Australia
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Kaspar Valgepea
3Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, Australia
4ERA Chair in Gas Fermentation Technologies, Institute of Technology, University of Tartu, Tartu, Estonia
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Ricardo A. Gonzalez-Garcia
3Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, Australia
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Colin Scott
2Synthetic Biology Future Science Platform, CSIRO, Sydney, Australia
5Biocatalysis and Synthetic Biology Team, CSIRO, Canberra, Australia
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Isak S. Pretorius
1Department of Molecular Sciences, Macquarie University, Sydney, Australia
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Esteban Marcellin
3Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, Australia
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Ian T. Paulsen
1Department of Molecular Sciences, Macquarie University, Sydney, Australia
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  • For correspondence: tom.williams@mq.edu.au ian.paulsen@mq.edu.au
Thomas C. Williams
1Department of Molecular Sciences, Macquarie University, Sydney, Australia
2Synthetic Biology Future Science Platform, CSIRO, Sydney, Australia
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  • For correspondence: tom.williams@mq.edu.au ian.paulsen@mq.edu.au
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Abstract

Microbial fermentation for chemical production is becoming more broadly adopted as an alternative to petrochemical refining. Fermentation typically relies on sugar as a feed-stock. However, one-carbon compounds like methanol are a more sustainable alternative as they do not compete with arable land. This study focused on engineering the capacity for methylotrophy in the yeast Saccharomyces cerevisiae through a yeast xylulose monophosphate (XuMP) pathway, a ‘hybrid’ XuMP pathway, and a bacterial ribulose monophosphate (RuMP) pathway. Through methanol toxicity assays and 13C-methanol-growth phenotypic characterization, the bacterial RuMP pathway was identified as the most promising synthetic pathway for methanol assimilation. When testing higher methanol concentrations, methanol assimilation was also observed in the wild-type strain, as 13C-ethanol was produced from 13C-methanol. These results demonstrate that S. cerevisiae has a previously undiscovered native capacity for methanol assimilation and pave the way for further development of both native and synthetic one-carbon assimilation pathways in S. cerevisiae.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted July 29, 2019.
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Native and synthetic methanol assimilation in Saccharomyces cerevisiae
Monica I. Espinosa, Kaspar Valgepea, Ricardo A. Gonzalez-Garcia, Colin Scott, Isak S. Pretorius, Esteban Marcellin, Ian T. Paulsen, Thomas C. Williams
bioRxiv 717942; doi: https://doi.org/10.1101/717942
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Native and synthetic methanol assimilation in Saccharomyces cerevisiae
Monica I. Espinosa, Kaspar Valgepea, Ricardo A. Gonzalez-Garcia, Colin Scott, Isak S. Pretorius, Esteban Marcellin, Ian T. Paulsen, Thomas C. Williams
bioRxiv 717942; doi: https://doi.org/10.1101/717942

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