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Genetic tool development in marine protists: Emerging model organisms for experimental cell biology

Drahomíra Faktorová, R. Ellen R. Nisbet, José A. Fernández Robledo, Elena Casacuberta, Lisa Sudek, Andrew E. Allen, Manuel Ares Jr., Cristina Aresté, Cecilia Balestreri, Adrian C. Barbrook, Patrick Beardslee, Sara Bender, David S. Booth, François-Yves Bouget, Chris Bowler, Susana A. Breglia, Anita Broellochs, Colin Brownlee, Gertraud Burger, Heriberto Cerutti, Rachele Cesaroni, Miguel A. Chiurillo, Thomas Clemente, Duncan B. Coles, Jackie L. Collier, Liz Cooney, Kathryn Coyne, Roberto Docampo, Christopher L. Dupont, Virginia Edgcomb, Elin Einarsson, Pía A. Elustondo, Fernan Federici, Veronica Freire-Beneitez, Nastasia J. Freyria, Kodai Fukuda, Paulo A. García, Peter R. Girguis, Fatma Gomaa, Sebastian Gornik, Jian Guo, Vladimír Hampl, Yutaka Hanawa, Esteban R. Haro-Contreras, Elisabeth Hehenberger, Andrea Highfield, Yoshihisa Hirakawa, Amanda Hopes, Christopher J. Howe, Ian Hu, Jorge Ibañez, Nicholas A.T. Irwin, Yuu Ishii, Natalia Ewa Janowicz, Adam C. Jones, Ambar Kachale, Konomi Fujimura-Kamada, Binnypreet Kaur, Jonathan Z. Kaye, Eleanna Kazana, Patrick J. Keeling, Nicole King, Lawrence A. Klobutcher, Noelia Lander, Imen Lassadi, Zhuhong Li, Senjie Lin, Jean-Claude Lozano, Fulei Luan, Shinichiro Maruyama, Tamara Matute, Cristina Miceli, Jun Minagawa, Mark Moosburner, Sebastián R. Najle, Deepak Nanjappa, Isabel C. Nimmo, Luke Noble, Anna M.G. Novák Vanclová, Mariusz Nowacki, Isaac Nuñez, Arnab Pain, Angela Piersanti, Sandra Pucciarelli, Jan Pyrih, Joshua S. Rest, Mariana Rius, Deborah Robertson, Aldane Ruaud, Iñaki Ruiz-Trillo, Monika A. Sigg, Pamela A. Silver, Claudio H. Slamovits, Estienne Swart, G. Jason Smith, Brittany Sprecher, Rowena Stern, Tobias von der Haar, Lev Tsypin, Aaron Turkewitz, Jernej Turnšek, Matus Valach, Valérie Vergé, Peter von Dassow, Anastasios Tsaousis, Ross F. Waller, Lu Wang, Xiaoxue Wen, Glen Wheeler, April Woods, Huan Zhang, Thomas Mock, Alexandra Z. Worden, Julius Lukeš
doi: https://doi.org/10.1101/718239
Drahomíra Faktorová
1Institute of Parasitology, Biology Centre, Czech Academy of Sciences and Faculty of Sciences, University of South Bohemia, České Budějovice, Czech Republic
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  • For correspondence: dranov@paru.cas.cz t.mock@uea.ac.uk azworden@geomar.de jula@paru.cas.cz
R. Ellen R. Nisbet
2Department of Biochemistry, University of Cambridge, Cambridge, UK
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José A. Fernández Robledo
3Bigelow Laboratory for Ocean Sciences, East Boothbay, USA
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Elena Casacuberta
4Institut de Biologia Evolutiva, CSIC-Universitat Pompeu Fabra, Barcelona, Spain
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Lisa Sudek
5Monterey Bay Aquarium Research Institute, Moss Landing, USA
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Andrew E. Allen
6Integrative Oceanography Division, Scripps Institution of Oceanography, University of California, San Diego, USA
7Microbial and Environmental Genomics, J. Craig Venter Institute, La Jolla, USA
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Manuel Ares Jr.
8Molecular, Cell and Developmental Biology, University of California, Santa Cruz, USA
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Cristina Aresté
4Institut de Biologia Evolutiva, CSIC-Universitat Pompeu Fabra, Barcelona, Spain
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Cecilia Balestreri
9The Marine Biological Association, Plymouth, UK
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Adrian C. Barbrook
2Department of Biochemistry, University of Cambridge, Cambridge, UK
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Patrick Beardslee
10School of Biological Sciences, University of Nebraska, Lincoln, USA
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Sara Bender
11Gordon and Betty Moore Foundation, Palo Alto, USA
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David S. Booth
12Department of Molecular and Cell Biology, University of California, Berkeley, USA
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François-Yves Bouget
13Sorbonne Université, CNRS UMR7621, Observatoire Océanologique, Banyul sur Mer, France
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Chris Bowler
14Institute de Biologie de l’ENS, Département de biologie, École Normale Supérieure, CNRS, INSERM, Paris, France
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Susana A. Breglia
15Centre for Comparative Genomics and Evolutionary Bioinformatics, Dalhousie University, Halifax, Canada
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Anita Broellochs
12Department of Molecular and Cell Biology, University of California, Berkeley, USA
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Colin Brownlee
9The Marine Biological Association, Plymouth, UK
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Gertraud Burger
16Department of Biochemistry and Robert-Cedergren Centre for Bioinformatics and Genomics, Université de Montréal, Montreal, Canada
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Heriberto Cerutti
10School of Biological Sciences, University of Nebraska, Lincoln, USA
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Rachele Cesaroni
17Institute of Cell Biology, University of Bern, Bern, Switzerland
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Miguel A. Chiurillo
18Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, USA
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Thomas Clemente
10School of Biological Sciences, University of Nebraska, Lincoln, USA
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Duncan B. Coles
3Bigelow Laboratory for Ocean Sciences, East Boothbay, USA
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Jackie L. Collier
19School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, USA
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Liz Cooney
20Department of Botany, University of British Columbia, Vancouver, Canada
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Kathryn Coyne
21University of Delaware College of Earth, Ocean and Environment, Lewes, USA
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Roberto Docampo
18Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, USA
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Christopher L. Dupont
7Microbial and Environmental Genomics, J. Craig Venter Institute, La Jolla, USA
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Virginia Edgcomb
22Woods Hole Oceanographic Institution, Woods Hole, MA, USA
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Elin Einarsson
2Department of Biochemistry, University of Cambridge, Cambridge, UK
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Pía A. Elustondo
15Centre for Comparative Genomics and Evolutionary Bioinformatics, Dalhousie University, Halifax, Canada
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Fernan Federici
23Facultad Ciencias Biológicas, Pontificia Universidad Católica de Chile, Fondo de Desarrollo de Areas Prioritarias, Center for Genome Regulation and Millennium Institute for Integrative Biology, Santiago de Chile, Chile
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Veronica Freire-Beneitez
24School of Biosciences, University of Kent, Canterbury, Kent, UK
25Laboratory of Molecular and Evolutionary Parasitology, University of Kent, UK
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Nastasia J. Freyria
3Bigelow Laboratory for Ocean Sciences, East Boothbay, USA
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Kodai Fukuda
26Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan
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Paulo A. García
27Department of Mechanical Engineering, Massachusetts Institute of Technology, Boston, USA
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Peter R. Girguis
28Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, USA
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Fatma Gomaa
28Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, USA
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Sebastian Gornik
29Centre for Organismal Studies, University of Heidelberg, Heidelberg, Germany
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Jian Guo
5Monterey Bay Aquarium Research Institute, Moss Landing, USA
8Molecular, Cell and Developmental Biology, University of California, Santa Cruz, USA
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Vladimír Hampl
30Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Vestec, Czech Republic
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Yutaka Hanawa
31Faculty of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan
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Esteban R. Haro-Contreras
15Centre for Comparative Genomics and Evolutionary Bioinformatics, Dalhousie University, Halifax, Canada
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Elisabeth Hehenberger
20Department of Botany, University of British Columbia, Vancouver, Canada
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Andrea Highfield
9The Marine Biological Association, Plymouth, UK
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Yoshihisa Hirakawa
31Faculty of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan
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Amanda Hopes
32School of Environmental Sciences, University of East Anglia, Norwich, UK
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Christopher J. Howe
2Department of Biochemistry, University of Cambridge, Cambridge, UK
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Ian Hu
2Department of Biochemistry, University of Cambridge, Cambridge, UK
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Jorge Ibañez
23Facultad Ciencias Biológicas, Pontificia Universidad Católica de Chile, Fondo de Desarrollo de Areas Prioritarias, Center for Genome Regulation and Millennium Institute for Integrative Biology, Santiago de Chile, Chile
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Nicholas A.T. Irwin
20Department of Botany, University of British Columbia, Vancouver, Canada
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Yuu Ishii
33Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan
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Natalia Ewa Janowicz
30Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Vestec, Czech Republic
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Adam C. Jones
11Gordon and Betty Moore Foundation, Palo Alto, USA
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Ambar Kachale
1Institute of Parasitology, Biology Centre, Czech Academy of Sciences and Faculty of Sciences, University of South Bohemia, České Budějovice, Czech Republic
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Konomi Fujimura-Kamada
34Division of Environmental Photobiology, National Institute for Basic Biology, Okazaki, Aichi, Japan
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Binnypreet Kaur
1Institute of Parasitology, Biology Centre, Czech Academy of Sciences and Faculty of Sciences, University of South Bohemia, České Budějovice, Czech Republic
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Jonathan Z. Kaye
11Gordon and Betty Moore Foundation, Palo Alto, USA
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Eleanna Kazana
24School of Biosciences, University of Kent, Canterbury, Kent, UK
25Laboratory of Molecular and Evolutionary Parasitology, University of Kent, UK
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Patrick J. Keeling
20Department of Botany, University of British Columbia, Vancouver, Canada
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Nicole King
12Department of Molecular and Cell Biology, University of California, Berkeley, USA
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Lawrence A. Klobutcher
35Department of Marine Sciences, University of Connecticut, Groton, USA
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Noelia Lander
18Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, USA
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Imen Lassadi
2Department of Biochemistry, University of Cambridge, Cambridge, UK
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Zhuhong Li
18Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, USA
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Senjie Lin
35Department of Marine Sciences, University of Connecticut, Groton, USA
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Jean-Claude Lozano
13Sorbonne Université, CNRS UMR7621, Observatoire Océanologique, Banyul sur Mer, France
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Fulei Luan
10School of Biological Sciences, University of Nebraska, Lincoln, USA
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Shinichiro Maruyama
33Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan
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Tamara Matute
23Facultad Ciencias Biológicas, Pontificia Universidad Católica de Chile, Fondo de Desarrollo de Areas Prioritarias, Center for Genome Regulation and Millennium Institute for Integrative Biology, Santiago de Chile, Chile
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Cristina Miceli
36School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy
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Jun Minagawa
34Division of Environmental Photobiology, National Institute for Basic Biology, Okazaki, Aichi, Japan
37Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies, Okazaki, Aichi, Japan
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Mark Moosburner
6Integrative Oceanography Division, Scripps Institution of Oceanography, University of California, San Diego, USA
7Microbial and Environmental Genomics, J. Craig Venter Institute, La Jolla, USA
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Sebastián R. Najle
4Institut de Biologia Evolutiva, CSIC-Universitat Pompeu Fabra, Barcelona, Spain
38Instituto de Biología Molecular y Celular, Universidad Nacional de Rosario, Rosario, Argentina
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Deepak Nanjappa
21University of Delaware College of Earth, Ocean and Environment, Lewes, USA
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Isabel C. Nimmo
2Department of Biochemistry, University of Cambridge, Cambridge, UK
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Luke Noble
39Center for Genomics and Systems Biology, New York University, New York, USA
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Anna M.G. Novák Vanclová
30Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Vestec, Czech Republic
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Mariusz Nowacki
17Institute of Cell Biology, University of Bern, Bern, Switzerland
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Isaac Nuñez
23Facultad Ciencias Biológicas, Pontificia Universidad Católica de Chile, Fondo de Desarrollo de Areas Prioritarias, Center for Genome Regulation and Millennium Institute for Integrative Biology, Santiago de Chile, Chile
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Arnab Pain
40Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Jeddah, Saudi Arabia
41Center for Zoonosis Control, Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo, Japan
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Angela Piersanti
36School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy
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Sandra Pucciarelli
36School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy
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Jan Pyrih
1Institute of Parasitology, Biology Centre, Czech Academy of Sciences and Faculty of Sciences, University of South Bohemia, České Budějovice, Czech Republic
24School of Biosciences, University of Kent, Canterbury, Kent, UK
30Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Vestec, Czech Republic
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Joshua S. Rest
42Department of Ecology and Evolution, Stony Brook University, Stony Brook, USA
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Mariana Rius
19School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, USA
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Deborah Robertson
43Lasry Center for Biosciences, Clark University, Worcester, USA
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Aldane Ruaud
23Facultad Ciencias Biológicas, Pontificia Universidad Católica de Chile, Fondo de Desarrollo de Areas Prioritarias, Center for Genome Regulation and Millennium Institute for Integrative Biology, Santiago de Chile, Chile
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Iñaki Ruiz-Trillo
4Institut de Biologia Evolutiva, CSIC-Universitat Pompeu Fabra, Barcelona, Spain
44Departament de Genètica Microbiologia i Estadiśtica, Universitat de Barcelona, Barcelona, Catalonia, Spain
45Catalan Institution for Research and Advanced Studies, Barcelona, Catalonia, Spain
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Monika A. Sigg
12Department of Molecular and Cell Biology, University of California, Berkeley, USA
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Pamela A. Silver
46Department of Systems Biology, Harvard Medical School, Boston, USA
47Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, USA
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Claudio H. Slamovits
15Centre for Comparative Genomics and Evolutionary Bioinformatics, Dalhousie University, Halifax, Canada
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Estienne Swart
17Institute of Cell Biology, University of Bern, Bern, Switzerland
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G. Jason Smith
48Department of Environmental Biotechnology, Moss Landing Marine Laboratoriess, Moss Landing, USA
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Brittany Sprecher
35Department of Marine Sciences, University of Connecticut, Groton, USA
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Rowena Stern
9The Marine Biological Association, Plymouth, UK
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Tobias von der Haar
24School of Biosciences, University of Kent, Canterbury, Kent, UK
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Lev Tsypin
49Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, USA
50Department of Biology, California Institute of Technology, Pasadena, USA
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Aaron Turkewitz
49Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, USA
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Jernej Turnšek
6Integrative Oceanography Division, Scripps Institution of Oceanography, University of California, San Diego, USA
7Microbial and Environmental Genomics, J. Craig Venter Institute, La Jolla, USA
46Department of Systems Biology, Harvard Medical School, Boston, USA
45Catalan Institution for Research and Advanced Studies, Barcelona, Catalonia, Spain
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Matus Valach
16Department of Biochemistry and Robert-Cedergren Centre for Bioinformatics and Genomics, Université de Montréal, Montreal, Canada
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Valérie Vergé
13Sorbonne Université, CNRS UMR7621, Observatoire Océanologique, Banyul sur Mer, France
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Peter von Dassow
23Facultad Ciencias Biológicas, Pontificia Universidad Católica de Chile, Fondo de Desarrollo de Areas Prioritarias, Center for Genome Regulation and Millennium Institute for Integrative Biology, Santiago de Chile, Chile
51Instituto Milenio de Oceanografia de Chile, Santiago de Chile, Chile
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Anastasios Tsaousis
24School of Biosciences, University of Kent, Canterbury, Kent, UK
25Laboratory of Molecular and Evolutionary Parasitology, University of Kent, UK
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Ross F. Waller
2Department of Biochemistry, University of Cambridge, Cambridge, UK
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Lu Wang
52Institute of Oceanography, Minjiang University, Fuzou, China
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Xiaoxue Wen
10School of Biological Sciences, University of Nebraska, Lincoln, USA
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Glen Wheeler
9The Marine Biological Association, Plymouth, UK
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April Woods
48Department of Environmental Biotechnology, Moss Landing Marine Laboratoriess, Moss Landing, USA
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Huan Zhang
35Department of Marine Sciences, University of Connecticut, Groton, USA
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Thomas Mock
32School of Environmental Sciences, University of East Anglia, Norwich, UK
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  • For correspondence: dranov@paru.cas.cz t.mock@uea.ac.uk azworden@geomar.de jula@paru.cas.cz
Alexandra Z. Worden
5Monterey Bay Aquarium Research Institute, Moss Landing, USA
53Ocean EcoSystems Biology Unit, Marine Ecology Division, Helmholtz Centre for Ocean Research, Kiel, Germany
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  • For correspondence: dranov@paru.cas.cz t.mock@uea.ac.uk azworden@geomar.de jula@paru.cas.cz
Julius Lukeš
1Institute of Parasitology, Biology Centre, Czech Academy of Sciences and Faculty of Sciences, University of South Bohemia, České Budějovice, Czech Republic
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ABSTRACT

Marine microbial eukaryotes underpin the largest food web on the planet and influence global biogeochemical cycles that maintain habitability. They are also remarkably diverse and provide insights into evolution, including the origins of complex life forms, as revealed through genome analyses. However, their genetic tractability has been limited to a few species that do not represent the broader diversity of eukaryotic life or some of the most environmentally relevant taxa. Here, we report on genetic systems developed as a community resource for experimental cell biology of marine protists from across the eukaryotic tree of life. We outline DNA delivery methods, expression constructs, and genome editing approaches that proved successful, as well as results from taxa where a working system was not achieved. The reported breakthroughs on genetic manipulation position the community to dissect cellular mechanisms from a breadth of protists, which will collectively provide insights into ancestral eukaryotic lifeforms, protein diversification and evolution of cellular pathways.

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Posted August 01, 2019.
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Genetic tool development in marine protists: Emerging model organisms for experimental cell biology
Drahomíra Faktorová, R. Ellen R. Nisbet, José A. Fernández Robledo, Elena Casacuberta, Lisa Sudek, Andrew E. Allen, Manuel Ares Jr., Cristina Aresté, Cecilia Balestreri, Adrian C. Barbrook, Patrick Beardslee, Sara Bender, David S. Booth, François-Yves Bouget, Chris Bowler, Susana A. Breglia, Anita Broellochs, Colin Brownlee, Gertraud Burger, Heriberto Cerutti, Rachele Cesaroni, Miguel A. Chiurillo, Thomas Clemente, Duncan B. Coles, Jackie L. Collier, Liz Cooney, Kathryn Coyne, Roberto Docampo, Christopher L. Dupont, Virginia Edgcomb, Elin Einarsson, Pía A. Elustondo, Fernan Federici, Veronica Freire-Beneitez, Nastasia J. Freyria, Kodai Fukuda, Paulo A. García, Peter R. Girguis, Fatma Gomaa, Sebastian Gornik, Jian Guo, Vladimír Hampl, Yutaka Hanawa, Esteban R. Haro-Contreras, Elisabeth Hehenberger, Andrea Highfield, Yoshihisa Hirakawa, Amanda Hopes, Christopher J. Howe, Ian Hu, Jorge Ibañez, Nicholas A.T. Irwin, Yuu Ishii, Natalia Ewa Janowicz, Adam C. Jones, Ambar Kachale, Konomi Fujimura-Kamada, Binnypreet Kaur, Jonathan Z. Kaye, Eleanna Kazana, Patrick J. Keeling, Nicole King, Lawrence A. Klobutcher, Noelia Lander, Imen Lassadi, Zhuhong Li, Senjie Lin, Jean-Claude Lozano, Fulei Luan, Shinichiro Maruyama, Tamara Matute, Cristina Miceli, Jun Minagawa, Mark Moosburner, Sebastián R. Najle, Deepak Nanjappa, Isabel C. Nimmo, Luke Noble, Anna M.G. Novák Vanclová, Mariusz Nowacki, Isaac Nuñez, Arnab Pain, Angela Piersanti, Sandra Pucciarelli, Jan Pyrih, Joshua S. Rest, Mariana Rius, Deborah Robertson, Aldane Ruaud, Iñaki Ruiz-Trillo, Monika A. Sigg, Pamela A. Silver, Claudio H. Slamovits, Estienne Swart, G. Jason Smith, Brittany Sprecher, Rowena Stern, Tobias von der Haar, Lev Tsypin, Aaron Turkewitz, Jernej Turnšek, Matus Valach, Valérie Vergé, Peter von Dassow, Anastasios Tsaousis, Ross F. Waller, Lu Wang, Xiaoxue Wen, Glen Wheeler, April Woods, Huan Zhang, Thomas Mock, Alexandra Z. Worden, Julius Lukeš
bioRxiv 718239; doi: https://doi.org/10.1101/718239
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Genetic tool development in marine protists: Emerging model organisms for experimental cell biology
Drahomíra Faktorová, R. Ellen R. Nisbet, José A. Fernández Robledo, Elena Casacuberta, Lisa Sudek, Andrew E. Allen, Manuel Ares Jr., Cristina Aresté, Cecilia Balestreri, Adrian C. Barbrook, Patrick Beardslee, Sara Bender, David S. Booth, François-Yves Bouget, Chris Bowler, Susana A. Breglia, Anita Broellochs, Colin Brownlee, Gertraud Burger, Heriberto Cerutti, Rachele Cesaroni, Miguel A. Chiurillo, Thomas Clemente, Duncan B. Coles, Jackie L. Collier, Liz Cooney, Kathryn Coyne, Roberto Docampo, Christopher L. Dupont, Virginia Edgcomb, Elin Einarsson, Pía A. Elustondo, Fernan Federici, Veronica Freire-Beneitez, Nastasia J. Freyria, Kodai Fukuda, Paulo A. García, Peter R. Girguis, Fatma Gomaa, Sebastian Gornik, Jian Guo, Vladimír Hampl, Yutaka Hanawa, Esteban R. Haro-Contreras, Elisabeth Hehenberger, Andrea Highfield, Yoshihisa Hirakawa, Amanda Hopes, Christopher J. Howe, Ian Hu, Jorge Ibañez, Nicholas A.T. Irwin, Yuu Ishii, Natalia Ewa Janowicz, Adam C. Jones, Ambar Kachale, Konomi Fujimura-Kamada, Binnypreet Kaur, Jonathan Z. Kaye, Eleanna Kazana, Patrick J. Keeling, Nicole King, Lawrence A. Klobutcher, Noelia Lander, Imen Lassadi, Zhuhong Li, Senjie Lin, Jean-Claude Lozano, Fulei Luan, Shinichiro Maruyama, Tamara Matute, Cristina Miceli, Jun Minagawa, Mark Moosburner, Sebastián R. Najle, Deepak Nanjappa, Isabel C. Nimmo, Luke Noble, Anna M.G. Novák Vanclová, Mariusz Nowacki, Isaac Nuñez, Arnab Pain, Angela Piersanti, Sandra Pucciarelli, Jan Pyrih, Joshua S. Rest, Mariana Rius, Deborah Robertson, Aldane Ruaud, Iñaki Ruiz-Trillo, Monika A. Sigg, Pamela A. Silver, Claudio H. Slamovits, Estienne Swart, G. Jason Smith, Brittany Sprecher, Rowena Stern, Tobias von der Haar, Lev Tsypin, Aaron Turkewitz, Jernej Turnšek, Matus Valach, Valérie Vergé, Peter von Dassow, Anastasios Tsaousis, Ross F. Waller, Lu Wang, Xiaoxue Wen, Glen Wheeler, April Woods, Huan Zhang, Thomas Mock, Alexandra Z. Worden, Julius Lukeš
bioRxiv 718239; doi: https://doi.org/10.1101/718239

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