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TALE and NF-Y co-occupancy marks enhancers of developmental control genes during zygotic genome activation in zebrafish

William Stanney III, Franck Ladam, Ian J. Donaldson, Teagan J. Parsons, René Maehr, Nicoletta Bobola, View ORCID ProfileCharles G Sagerström
doi: https://doi.org/10.1101/720102
William Stanney III
1Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
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Franck Ladam
1Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
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Ian J. Donaldson
3Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK
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Teagan J. Parsons
2Program in Molecular Medicine and Diabetes Center of Excellence, University of Massachusetts Medical School, Worcester, MA 01605, USA
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René Maehr
2Program in Molecular Medicine and Diabetes Center of Excellence, University of Massachusetts Medical School, Worcester, MA 01605, USA
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Nicoletta Bobola
3Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK
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Charles G Sagerström
1Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
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  • ORCID record for Charles G Sagerström
  • For correspondence: charles.sagerstrom@ucdenver.edu
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SUMMARY

Animal embryogenesis is initiated by maternal factors, but zygotic genome activation (ZGA) shifts control to the embryo at early blastula stages. ZGA is thought to be mediated by specialized maternally deposited transcription factors (TFs), but here we demonstrate that NF-Y and TALE – TFs with known later roles in embryogenesis – co-occupy unique genomic elements at zebrafish ZGA. We show that these elements are selectively associated with early-expressed genes involved in transcriptional regulation and possess enhancer activity in vivo. In contrast, we find that elements individually occupied by either NF-Y or TALE are associated with genes acting later in development – such that NF-Y controls a cilia gene expression program while TALE TFs control expression of hox genes. We conclude that NF-Y and TALE have a shared role at ZGA, but separate roles later during development, demonstrating that combinations of known TFs can regulate subsets of key developmental genes at vertebrate ZGA.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted July 31, 2019.
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TALE and NF-Y co-occupancy marks enhancers of developmental control genes during zygotic genome activation in zebrafish
William Stanney III, Franck Ladam, Ian J. Donaldson, Teagan J. Parsons, René Maehr, Nicoletta Bobola, Charles G Sagerström
bioRxiv 720102; doi: https://doi.org/10.1101/720102
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TALE and NF-Y co-occupancy marks enhancers of developmental control genes during zygotic genome activation in zebrafish
William Stanney III, Franck Ladam, Ian J. Donaldson, Teagan J. Parsons, René Maehr, Nicoletta Bobola, Charles G Sagerström
bioRxiv 720102; doi: https://doi.org/10.1101/720102

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