Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Visualising nascent chain dynamics at the ribosome exit tunnel by cryo-electron microscopy

Abid Javed, Tomasz Wlodarski, Anaïs. M.E. Cassaignau, Lisa D. Cabrita, John Christodoulou, Elena V. Orlova
doi: https://doi.org/10.1101/722611
Abid Javed
1Institute of Structural and Molecular Biology, Birkbeck College, Malet Street, London WC1E 7HX, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tomasz Wlodarski
2Institute of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anaïs. M.E. Cassaignau
2Institute of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lisa D. Cabrita
2Institute of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
John Christodoulou
2Institute of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: j.christodoulou@ucl.ac.uk e.orlova@mail.cryst.bbk.ac.uk
Elena V. Orlova
1Institute of Structural and Molecular Biology, Birkbeck College, Malet Street, London WC1E 7HX, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: j.christodoulou@ucl.ac.uk e.orlova@mail.cryst.bbk.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Ribosomes maintain a healthy cellular proteome by synthesising proteins. The nascent chain (NC), emerges into the cellular milieu via the ribosomal exit tunnel, which is an active component that regulates the NC passage. How the NC dynamics at the exit tunnel affect NC folding remains to be an important question, the answer on which has strong implications to medicine. Here, we report high-resolution cryo-EM maps of ribosome nascent-chain complexes (RNCs) displaying distinct steps during biosynthesis. These RNC structures reveal a range of pathways adopted by the NC. The most pronounced diversity in the NC trajectories were found in the vestibule region. Rearrangements of several ribosomal components further suggest that these elements may actively monitor the emerging NC during translation. The ribosome-NC contacts within the vestibule define these NC pathways and modulate position of a folded immunoglobulin domain outside the ribosome.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted December 16, 2019.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Visualising nascent chain dynamics at the ribosome exit tunnel by cryo-electron microscopy
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Visualising nascent chain dynamics at the ribosome exit tunnel by cryo-electron microscopy
Abid Javed, Tomasz Wlodarski, Anaïs. M.E. Cassaignau, Lisa D. Cabrita, John Christodoulou, Elena V. Orlova
bioRxiv 722611; doi: https://doi.org/10.1101/722611
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Visualising nascent chain dynamics at the ribosome exit tunnel by cryo-electron microscopy
Abid Javed, Tomasz Wlodarski, Anaïs. M.E. Cassaignau, Lisa D. Cabrita, John Christodoulou, Elena V. Orlova
bioRxiv 722611; doi: https://doi.org/10.1101/722611

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biophysics
Subject Areas
All Articles
  • Animal Behavior and Cognition (4654)
  • Biochemistry (10299)
  • Bioengineering (7614)
  • Bioinformatics (26189)
  • Biophysics (13446)
  • Cancer Biology (10620)
  • Cell Biology (15333)
  • Clinical Trials (138)
  • Developmental Biology (8452)
  • Ecology (12754)
  • Epidemiology (2067)
  • Evolutionary Biology (16763)
  • Genetics (11356)
  • Genomics (15400)
  • Immunology (10548)
  • Microbiology (25041)
  • Molecular Biology (10152)
  • Neuroscience (54096)
  • Paleontology (398)
  • Pathology (1655)
  • Pharmacology and Toxicology (2877)
  • Physiology (4314)
  • Plant Biology (9197)
  • Scientific Communication and Education (1579)
  • Synthetic Biology (2541)
  • Systems Biology (6752)
  • Zoology (1452)