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Structural diversity of oligomeric β-propellers with different numbers of identical blades

Evgenia Afanasieva, Indronil Chaudhuri, Jörg Martin, Eva Hertle, Astrid Ursinus, View ORCID ProfileVikram Alva, View ORCID ProfileMarcus D. Hartmann, View ORCID ProfileAndrei N. Lupas
doi: https://doi.org/10.1101/723619
Evgenia Afanasieva
Department of Protein Evolution, Max Planck Institute for Developmental Biology, Max-Planck-Ring 5, D-72076 Tübingen, Germany
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Indronil Chaudhuri
Department of Protein Evolution, Max Planck Institute for Developmental Biology, Max-Planck-Ring 5, D-72076 Tübingen, Germany
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Jörg Martin
Department of Protein Evolution, Max Planck Institute for Developmental Biology, Max-Planck-Ring 5, D-72076 Tübingen, Germany
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Eva Hertle
Department of Protein Evolution, Max Planck Institute for Developmental Biology, Max-Planck-Ring 5, D-72076 Tübingen, Germany
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Astrid Ursinus
Department of Protein Evolution, Max Planck Institute for Developmental Biology, Max-Planck-Ring 5, D-72076 Tübingen, Germany
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Vikram Alva
Department of Protein Evolution, Max Planck Institute for Developmental Biology, Max-Planck-Ring 5, D-72076 Tübingen, Germany
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  • ORCID record for Vikram Alva
Marcus D. Hartmann
Department of Protein Evolution, Max Planck Institute for Developmental Biology, Max-Planck-Ring 5, D-72076 Tübingen, Germany
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Andrei N. Lupas
Department of Protein Evolution, Max Planck Institute for Developmental Biology, Max-Planck-Ring 5, D-72076 Tübingen, Germany
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  • ORCID record for Andrei N. Lupas
  • For correspondence: andrei.lupas@tuebingen.mpg.de
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Abstract

β-Propellers arise through the amplification of a supersecondary structure element called a blade. This process produces toroids of between four and twelve repeats, which are almost always arranged sequentially in a single polypeptide chain. We found that new propellers evolve continuously by amplification from single blades. We therefore investigated whether such nascent propellers can fold as homo-oligomers before they have been fully amplified within a single chain. One-to six-bladed building blocks derived from two seven-bladed WD40 propellers yielded stable homo-oligomers with six to nine blades, depending on the size of the building block. High-resolution structures for tetramers of two blades, trimers of three blades, and dimers of four and five blades, respectively, show structurally diverse propellers and include a novel fold, highlighting the inherent flexibility of the WD40 blade. Our data support the hypothesis that subdomain-sized fragments can provide structural versatility in the evolution of new proteins.

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  • ↵† joint first authors

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted August 02, 2019.
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Structural diversity of oligomeric β-propellers with different numbers of identical blades
Evgenia Afanasieva, Indronil Chaudhuri, Jörg Martin, Eva Hertle, Astrid Ursinus, Vikram Alva, Marcus D. Hartmann, Andrei N. Lupas
bioRxiv 723619; doi: https://doi.org/10.1101/723619
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Structural diversity of oligomeric β-propellers with different numbers of identical blades
Evgenia Afanasieva, Indronil Chaudhuri, Jörg Martin, Eva Hertle, Astrid Ursinus, Vikram Alva, Marcus D. Hartmann, Andrei N. Lupas
bioRxiv 723619; doi: https://doi.org/10.1101/723619

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