Abstract
Precise regulation of gene expression in response to environmental changes is crucial for cell survival, adaptation and proliferation. In eukaryotic cells, extracellular signal integration is often carried out by Mitogen-Activated Protein Kinases (MAPK), which control cell differentiation, apoptosis and stress response. Despite a robust MAPK signaling activity, downstream gene expression can display a great variability between single cells. We have used Saccharomyces cerevisiae to study the dynamics of transcription of stress-responsive genes. Following a hyper-osmotic shock, the MAPK Hog1 induces the transcription of hundreds of genes. Using a PP7 reporter assay, we have monitored with high temporal resolution and in single cells, the dynamics of transcription from a set of promoters in order to highlight how the onset, the level and the shutoff of transcription are regulated in this model MAPK pathway.
Footnotes
Updated typo in the title