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Emergence and patterning dynamics of mouse definitive endoderm

Maayan Pour, Abhishek Sampath Kumar, Maria Walther, Lars Wittler, Alexander Meissner, Iftach Nachman
doi: https://doi.org/10.1101/728642
Maayan Pour
1Department of Biochemistry and Molecular Biology, Tel Aviv University, Tel Aviv, Israel
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Abhishek Sampath Kumar
2Department of Genome Regulation, Max Planck Institute for Molecular Genetics, Berlin 14195, Germany
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Maria Walther
2Department of Genome Regulation, Max Planck Institute for Molecular Genetics, Berlin 14195, Germany
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Lars Wittler
3Department of Developmental Genetics, Max Planck Institute for Molecular Genetics, Berlin 14195, Germany
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Alexander Meissner
2Department of Genome Regulation, Max Planck Institute for Molecular Genetics, Berlin 14195, Germany
4Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA
5Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA
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Iftach Nachman
1Department of Biochemistry and Molecular Biology, Tel Aviv University, Tel Aviv, Israel
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  • For correspondence: iftach.nachman@gmail.com
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Abstract

The segregation of definitive endoderm (DE) from mesendoderm progenitors leads to the formation of two distinct germ layers. Dissecting DE onset has been challenging as it occurs within a narrow spatio-temporal window in the embryo. Here we employ a dual Bra-GFP, Sox17-RFP reporter cell line to study DE onset dynamics. We find Sox17 starts in a few isolated cells in vivo. Using 2D and 3D in vitro models, we show that DE cells emerge from mesendoderm progenitors at a temporally regular, but spatially stochastic pattern, which is subsequently arranged by self-sorting of Sox17+ cells. Self-sorting coincides with up-regulation of E-cadherin but is not necessary for DE differentiation or proliferation. A subpopulation of Bra-high cells commits to a Sox17+ fate independent of external Wnt signal. Our in vivo and in vitro results highlight basic rules governing DE onset and patterning through the commonalities and differences between these systems.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted August 07, 2019.
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Emergence and patterning dynamics of mouse definitive endoderm
Maayan Pour, Abhishek Sampath Kumar, Maria Walther, Lars Wittler, Alexander Meissner, Iftach Nachman
bioRxiv 728642; doi: https://doi.org/10.1101/728642
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Emergence and patterning dynamics of mouse definitive endoderm
Maayan Pour, Abhishek Sampath Kumar, Maria Walther, Lars Wittler, Alexander Meissner, Iftach Nachman
bioRxiv 728642; doi: https://doi.org/10.1101/728642

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