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Predicting metabolism during growth by osmotic cell expansion

View ORCID ProfileSanu Shameer, View ORCID ProfileJosé G. Vallarino, View ORCID ProfileAlisdair R. Fernie, View ORCID ProfileR. George Ratcliffe, View ORCID ProfileLee J Sweetlove
doi: https://doi.org/10.1101/731232
Sanu Shameer
1Department of Plant Sciences, University of Oxford, Oxford, UK
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José G. Vallarino
2Max Planck Institute for Molecular Plant Physiology, Potsdam-Golm, Germany
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Alisdair R. Fernie
2Max Planck Institute for Molecular Plant Physiology, Potsdam-Golm, Germany
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R. George Ratcliffe
1Department of Plant Sciences, University of Oxford, Oxford, UK
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Lee J Sweetlove
1Department of Plant Sciences, University of Oxford, Oxford, UK
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  • For correspondence: lee.sweetlove@plants.ox.ac.uk
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ABSTRACT

Cell expansion is a significant contributor to organ growth and is driven by the accumulation of osmolytes to increase cell turgor pressure. Metabolic modelling has the potential to provide insights into the processes that underpin osmolyte synthesis and transport, but the main computational approach for predicting metabolic network fluxes, flux balance analysis (FBA), typically uses biomass composition as the main output constraint and ignores potential changes in cell volume. Here we present GrOE-FBA (Growth by Osmotic Expansion-Flux Balance Analysis), a framework that accounts for both the metabolic and ionic contributions to the osmotica that drive cell expansion, as well as the synthesis of protein, cell wall and cell membrane components required for cell enlargement. Using GrOE-FBA, the metabolic fluxes in dividing and expanding cell were analyzed, and the energetic costs for metabolite biosynthesis and accumulation in the two scenarios were found to be surprisingly similar. The expansion phase of tomato fruit growth was also modelled using a multi-phase single optimization GrOE-FBA model and this approach gave accurate predictions of the major metabolite levels throughout fruit development as well as revealing a role for transitory starch accumulation in ensuring optimal fruit development.

Footnotes

  • Author information page was section was missing two coauthors. Information was correctly presented in PDF and so manuscript is unchanged.

  • https://github.com/ljs1002/Shameer-et-al-Predicting-metabolism-during-growth-by-osmotic-cell-expansion

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted August 12, 2019.
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Predicting metabolism during growth by osmotic cell expansion
Sanu Shameer, José G. Vallarino, Alisdair R. Fernie, R. George Ratcliffe, Lee J Sweetlove
bioRxiv 731232; doi: https://doi.org/10.1101/731232
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Predicting metabolism during growth by osmotic cell expansion
Sanu Shameer, José G. Vallarino, Alisdair R. Fernie, R. George Ratcliffe, Lee J Sweetlove
bioRxiv 731232; doi: https://doi.org/10.1101/731232

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