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A Combinatorial MAP Code Dictates Polarized Microtubule Transport

Brigette Y. Monroy, Tracy C. Tan, Janah May Oclaman, Jisoo S. Han, Sergi Simo, Dan W. Nowakowski, Richard J. McKenney, Kassandra M. Ori-McKenney
doi: https://doi.org/10.1101/731604
Brigette Y. Monroy
1Department of Molecular and Cellular Biology, University of California, Davis, Davis, California, 95616
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Tracy C. Tan
1Department of Molecular and Cellular Biology, University of California, Davis, Davis, California, 95616
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Janah May Oclaman
1Department of Molecular and Cellular Biology, University of California, Davis, Davis, California, 95616
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Jisoo S. Han
2Dept. of Cell Biology and Human Anatomy, School of Medicine. University of California – Davis. 3402 Tupper Hall, Davis CA. 95616. USA
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Sergi Simo
2Dept. of Cell Biology and Human Anatomy, School of Medicine. University of California – Davis. 3402 Tupper Hall, Davis CA. 95616. USA
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Dan W. Nowakowski
3N Molecular Systems, Inc., Palo Alto, CA. 94303 USA
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Richard J. McKenney
1Department of Molecular and Cellular Biology, University of California, Davis, Davis, California, 95616
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Kassandra M. Ori-McKenney
1Department of Molecular and Cellular Biology, University of California, Davis, Davis, California, 95616
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  • For correspondence: kmorimckenney@ucdavis.edu
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ABSTRACT

Many eukaryotic cells distribute their intracellular components through asymmetrically regulated active transport driven by molecular motors along microtubule tracks. While intrinsic and extrinsic regulation of motor activity exists, what governs the overall distribution of activated motor-cargo complexes within cells remains unclear. Here, we utilize in vitro reconstitution of purified motor proteins and non-enzymatic microtubule-associated proteins (MAPs) to demonstrate that these MAPs exhibit distinct influences on the motility of the three main classes of transport motors: kinesin-1, kinesin-3, and cytoplasmic dynein. Further, we dissect how combinations of MAPs affect motors, and reveal how transient interactions between MAPs and motors may promote these effects. From these data, we propose a general “MAP code” that has the capacity to strongly bias directed movement along microtubules and helps elucidate the intricate intracellular sorting observed in highly polarized cells such as neurons.

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Posted August 10, 2019.
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A Combinatorial MAP Code Dictates Polarized Microtubule Transport
Brigette Y. Monroy, Tracy C. Tan, Janah May Oclaman, Jisoo S. Han, Sergi Simo, Dan W. Nowakowski, Richard J. McKenney, Kassandra M. Ori-McKenney
bioRxiv 731604; doi: https://doi.org/10.1101/731604
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A Combinatorial MAP Code Dictates Polarized Microtubule Transport
Brigette Y. Monroy, Tracy C. Tan, Janah May Oclaman, Jisoo S. Han, Sergi Simo, Dan W. Nowakowski, Richard J. McKenney, Kassandra M. Ori-McKenney
bioRxiv 731604; doi: https://doi.org/10.1101/731604

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