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Recruitment of mRNAs to P granules by gelation with intrinsically-disordered proteins

View ORCID ProfileChih-Yung S. Lee, Andrea Putnam, View ORCID ProfileTu Lu, Shuaixin He, View ORCID ProfileJohn Paul T. Ouyang, View ORCID ProfileGeraldine Seydoux
doi: https://doi.org/10.1101/732057
Chih-Yung S. Lee
1HHMI and Dept. of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore MD USA
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Andrea Putnam
1HHMI and Dept. of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore MD USA
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Tu Lu
1HHMI and Dept. of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore MD USA
3Google, 1600 Amphitheatre Parkway, Mountain View, CA, USA
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Shuaixin He
1HHMI and Dept. of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore MD USA
2Dept. of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore MD USA
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John Paul T. Ouyang
1HHMI and Dept. of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore MD USA
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Geraldine Seydoux
1HHMI and Dept. of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore MD USA
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  • ORCID record for Geraldine Seydoux
  • For correspondence: gseydoux@jhmi.edu
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Abstract

Animals with germ plasm assemble cytoplasmic RNA granules (germ granules) that segregate with the embryonic germ lineage. How germ granules assemble and recruit RNA is not well understood. Here we characterize the assembly and RNA composition of the germ (P) granules of C. elegans. ∼500 maternal mRNAs are recruited into P granules by a sequence independent mechanism that favors mRNAs with low ribosome coverage. Translational activation correlates temporally with P granule exit for two mRNAs that code for germ cell fate regulators. mRNAs are recruited into the granules by MEG-3, an intrinsically disordered protein that condenses with RNA to form nanoscale gels. Our observations reveal parallels between germ granules and stress granules and suggest that cytoplasmic RNA granules are reversible super-assemblies of nanoscale RNA-protein gel condensates.

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Posted August 11, 2019.
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Recruitment of mRNAs to P granules by gelation with intrinsically-disordered proteins
Chih-Yung S. Lee, Andrea Putnam, Tu Lu, Shuaixin He, John Paul T. Ouyang, Geraldine Seydoux
bioRxiv 732057; doi: https://doi.org/10.1101/732057
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Recruitment of mRNAs to P granules by gelation with intrinsically-disordered proteins
Chih-Yung S. Lee, Andrea Putnam, Tu Lu, Shuaixin He, John Paul T. Ouyang, Geraldine Seydoux
bioRxiv 732057; doi: https://doi.org/10.1101/732057

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