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DUX4 regulates oocyte to embryo transition in human

Sanna Vuoristo, Christel Hydén-Granskog, Masahito Yoshihara, Lisa Gawriyski, Anastassius Damdimopoulos, Shruti Bhagat, View ORCID ProfileKosuke Hashimoto, Kaarel Krjutškov, Sini Ezer, Priit Paluoja, Karolina Lundin, Pauliina Paloviita, Gaëlle Recher, Vipin Ranga, Tomi Airenne, Mahlet Tamirat, Eeva-Mari Jouhilahti, Timo Otonkoski, Juha S. Tapanainen, Hideya Kawaji, Yasuhiro Murakawa, Thomas R. Bürglin, Markku Varjosalo, Mark S. Johnson, Timo Tuuri, Shintaro Katayama, Juha Kere
doi: https://doi.org/10.1101/732289
Sanna Vuoristo
1Karolinska Institutet, Department of Biosciences and Nutrition, Huddinge, Sweden
2University of Helsinki, Department of Obstetrics and Gynecology, Helsinki, Finland
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  • For correspondence: juha.kere@ki.se
Christel Hydén-Granskog
3Helsinki University Hospital, Department of Obstetrics and Gynecology, Helsinki, Finland
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Masahito Yoshihara
1Karolinska Institutet, Department of Biosciences and Nutrition, Huddinge, Sweden
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Lisa Gawriyski
4University of Helsinki, Institute of Biotechnology, Helsinki, Finland
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Anastassius Damdimopoulos
5Karolinska Institutet, Bioinformatics and Expression Analysis Core Facility, Huddinge, Sweden
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Shruti Bhagat
1Karolinska Institutet, Department of Biosciences and Nutrition, Huddinge, Sweden
6RIKEN Center for Integrative Medical Sciences, Yokohama, Japan
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Kosuke Hashimoto
6RIKEN Center for Integrative Medical Sciences, Yokohama, Japan
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  • ORCID record for Kosuke Hashimoto
Kaarel Krjutškov
1Karolinska Institutet, Department of Biosciences and Nutrition, Huddinge, Sweden
7Competence Centre for Health Technologies, Tartu, Estonia
8Stem Cells and Metabolism Research Program, University of Helsinki, Finland
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Sini Ezer
8Stem Cells and Metabolism Research Program, University of Helsinki, Finland
9Folkhälsan Research Center, Helsinki, Finland
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Priit Paluoja
7Competence Centre for Health Technologies, Tartu, Estonia
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Karolina Lundin
2University of Helsinki, Department of Obstetrics and Gynecology, Helsinki, Finland
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Pauliina Paloviita
2University of Helsinki, Department of Obstetrics and Gynecology, Helsinki, Finland
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Gaëlle Recher
10Institut d’Optique Graduate School, CNRS - Université de Bordeaux, Talence, France
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Vipin Ranga
11Structural Bioinformatics Laboratory, Biochemistry, Faculty of Science and Engineering, Åbo Akademi University, Turku, Finland
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Tomi Airenne
11Structural Bioinformatics Laboratory, Biochemistry, Faculty of Science and Engineering, Åbo Akademi University, Turku, Finland
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Mahlet Tamirat
11Structural Bioinformatics Laboratory, Biochemistry, Faculty of Science and Engineering, Åbo Akademi University, Turku, Finland
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Eeva-Mari Jouhilahti
8Stem Cells and Metabolism Research Program, University of Helsinki, Finland
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Timo Otonkoski
8Stem Cells and Metabolism Research Program, University of Helsinki, Finland
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Juha S. Tapanainen
2University of Helsinki, Department of Obstetrics and Gynecology, Helsinki, Finland
3Helsinki University Hospital, Department of Obstetrics and Gynecology, Helsinki, Finland
12Oulu University Hospital, Oulu, Finland
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Hideya Kawaji
6RIKEN Center for Integrative Medical Sciences, Yokohama, Japan
13RIKEN Preventive Medicine and Diagnosis Innovation Program, Wako, Japan
14Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
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Yasuhiro Murakawa
6RIKEN Center for Integrative Medical Sciences, Yokohama, Japan
15IFOM, The FIRC Institute of Molecular Oncology, Milan, Italy
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Thomas R. Bürglin
16University of Basel, Department of Biomedicine, Basel, Switzerland
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Markku Varjosalo
4University of Helsinki, Institute of Biotechnology, Helsinki, Finland
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Mark S. Johnson
11Structural Bioinformatics Laboratory, Biochemistry, Faculty of Science and Engineering, Åbo Akademi University, Turku, Finland
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Timo Tuuri
2University of Helsinki, Department of Obstetrics and Gynecology, Helsinki, Finland
3Helsinki University Hospital, Department of Obstetrics and Gynecology, Helsinki, Finland
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Shintaro Katayama
1Karolinska Institutet, Department of Biosciences and Nutrition, Huddinge, Sweden
9Folkhälsan Research Center, Helsinki, Finland
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  • For correspondence: juha.kere@ki.se
Juha Kere
1Karolinska Institutet, Department of Biosciences and Nutrition, Huddinge, Sweden
8Stem Cells and Metabolism Research Program, University of Helsinki, Finland
9Folkhälsan Research Center, Helsinki, Finland
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  • For correspondence: juha.kere@ki.se
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Abstract

In oocyte to embryo transition, the fertilized oocyte undergoes final maturation and the embryo genome is gradually activated during the first three cell divisions. How this transition is coordinated and which factors drive the processes in humans is largely unknown. Here we studied the role of the double homeodomain transcription factor DUX4 in regulating the human oocyte to embryo transition. DUX4 knockdown zygotes show delayed transcriptome reprogramming during the first three days after fertilization. Our combined experimental approaches allowed integrated analysis on the transcriptome, chromatin, and proteome data in human embryos or a DUX4 expressing human embryonic stem cell model. We conclude that DUX4 is a pioneering factor that regulates human oocyte to embryo transition through regulating oocyte mRNA degradation, as well as direct binding and activation of minor genome activation genes, and genomic repeat elements.

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  • ↵# Current affiliation

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Posted August 12, 2019.
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DUX4 regulates oocyte to embryo transition in human
Sanna Vuoristo, Christel Hydén-Granskog, Masahito Yoshihara, Lisa Gawriyski, Anastassius Damdimopoulos, Shruti Bhagat, Kosuke Hashimoto, Kaarel Krjutškov, Sini Ezer, Priit Paluoja, Karolina Lundin, Pauliina Paloviita, Gaëlle Recher, Vipin Ranga, Tomi Airenne, Mahlet Tamirat, Eeva-Mari Jouhilahti, Timo Otonkoski, Juha S. Tapanainen, Hideya Kawaji, Yasuhiro Murakawa, Thomas R. Bürglin, Markku Varjosalo, Mark S. Johnson, Timo Tuuri, Shintaro Katayama, Juha Kere
bioRxiv 732289; doi: https://doi.org/10.1101/732289
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DUX4 regulates oocyte to embryo transition in human
Sanna Vuoristo, Christel Hydén-Granskog, Masahito Yoshihara, Lisa Gawriyski, Anastassius Damdimopoulos, Shruti Bhagat, Kosuke Hashimoto, Kaarel Krjutškov, Sini Ezer, Priit Paluoja, Karolina Lundin, Pauliina Paloviita, Gaëlle Recher, Vipin Ranga, Tomi Airenne, Mahlet Tamirat, Eeva-Mari Jouhilahti, Timo Otonkoski, Juha S. Tapanainen, Hideya Kawaji, Yasuhiro Murakawa, Thomas R. Bürglin, Markku Varjosalo, Mark S. Johnson, Timo Tuuri, Shintaro Katayama, Juha Kere
bioRxiv 732289; doi: https://doi.org/10.1101/732289

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