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Profiling of presynaptic mRNAs reveals a role for axonal PUMILIOs in associative memory formation

View ORCID ProfileRachel Arey, View ORCID ProfileRachel Kaletsky, View ORCID ProfileColeen T. Murphy
doi: https://doi.org/10.1101/733428
Rachel Arey
1Department of Molecular Biology, Princeton University, Princeton NJ 08544
2LSI Genomics, Princeton University, Princeton NJ 08544
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  • ORCID record for Rachel Arey
Rachel Kaletsky
1Department of Molecular Biology, Princeton University, Princeton NJ 08544
2LSI Genomics, Princeton University, Princeton NJ 08544
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Coleen T. Murphy
1Department of Molecular Biology, Princeton University, Princeton NJ 08544
2LSI Genomics, Princeton University, Princeton NJ 08544
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  • For correspondence: ctmurphy@princeton.edu
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Abstract

Presynaptic protein synthesis is important in the adult central nervous system; however, the set of mRNAs localized to presynaptic areas has yet to be identified in any organism. We differentially labeled somatic and synaptic compartments nervous system-wide in adult C. elegans and isolated synaptic regions for deep sequencing. Analysis of the synaptic transcriptome reveals that synaptic transcripts are predicted to have specialized functions in neurons. Differential expression analysis identified 543 genes enriched in synaptic regions relative to somatic regions, with synaptic functions conserved in higher organisms. We find that mRNAs for pumilio RNA-binding proteins are abundant in synaptic regions, which we confirmed through high-sensitivity in situ hybridization. We identified a new role for the PUM2 orthologs puf-7/8 as repressors of memory formation through regulation of the mitochondrial dynamics regulator, mff-1. Identification of presynaptic mRNAs provides insight into mechanisms that regulate synaptic function and behavior.

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Posted August 13, 2019.
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Profiling of presynaptic mRNAs reveals a role for axonal PUMILIOs in associative memory formation
Rachel Arey, Rachel Kaletsky, Coleen T. Murphy
bioRxiv 733428; doi: https://doi.org/10.1101/733428
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Profiling of presynaptic mRNAs reveals a role for axonal PUMILIOs in associative memory formation
Rachel Arey, Rachel Kaletsky, Coleen T. Murphy
bioRxiv 733428; doi: https://doi.org/10.1101/733428

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