Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Hedgehog signaling regulates neurogenesis in the larval and adult zebrafish hypothalamus

Ira Male, A. Tuba Ozacar, Rita R. Fagan, Matthew Loring, Meng-Chieh Shen, Veronica Pace, Chris Devine, Alyssa Lutservitz, Rolf O. Karlstrom
doi: https://doi.org/10.1101/740613
Ira Male
Department of Biology, University of Massachusetts, Amherst, MA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
A. Tuba Ozacar
Department of Biology, University of Massachusetts, Amherst, MAHisar Schools, Istanbul, Turkey
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rita R. Fagan
Department of Biology, University of Massachusetts, Amherst, MADept. of Neurobiology, Univ. of Massachusetts Medical School, Worcester, MA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Matthew Loring
Department of Biology, University of Massachusetts, Amherst, MADept. of Neurobiology, Duke University School of Medicine, Durham, NC
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Meng-Chieh Shen
Department of Biology, University of Massachusetts, Amherst, MADept. of Embryology, Carnegie Institution for Science, Baltimore, MD
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Veronica Pace
Department of Biology, University of Massachusetts, Amherst, MADept. of Molecular Medicine, Univ. of Massachusetts Medical School, Worcester, MA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chris Devine
Department of Biology, University of Massachusetts, Amherst, MAQueensland University of Technology, Brisbane, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alyssa Lutservitz
Department of Biology, University of Massachusetts, Amherst, MADept. of Genetics, Harvard Medical School, Boston, MA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rolf O. Karlstrom
Department of Biology, University of Massachusetts, Amherst, MA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: karlstrom@bio.umass.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

While neurogenesis in the adult hypothalamus is now known to be essential for proper function, the cell-cell signaling events that regulate neurogenesis in this evolutionarily conserved brain region remain poorly understood. Here we show that Hedgehog (Hh)/Gli signaling positively regulates hypothalamic neurogenesis in both larval and adult zebrafish and is necessary and sufficient for normal hypothalamic proliferation rates. Hedgehog-responsive cells are relatively rapidly proliferating pluripotent neural precursors that give rise to dopaminergic, serotonergic, and GABAergic neurons. in situ and transgenic reporter analyses revealed substantial heterogeneity in cell-cell signaling within the hypothalamic niche, with slow cycling Nestin-expressing cells residing among distinct and overlapping populations of Sonic Hh (Shh)-expressing, Hh-responsive, Notch-responsive, and Wnt-responsive radial glia. This work shows for the first time that Hh/Gli-signaling is a key component of the complex cell-cell signaling environment that regulates hypothalamic neurogenesis throughout life.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted August 26, 2019.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Hedgehog signaling regulates neurogenesis in the larval and adult zebrafish hypothalamus
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
Share
Hedgehog signaling regulates neurogenesis in the larval and adult zebrafish hypothalamus
Ira Male, A. Tuba Ozacar, Rita R. Fagan, Matthew Loring, Meng-Chieh Shen, Veronica Pace, Chris Devine, Alyssa Lutservitz, Rolf O. Karlstrom
bioRxiv 740613; doi: https://doi.org/10.1101/740613
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Hedgehog signaling regulates neurogenesis in the larval and adult zebrafish hypothalamus
Ira Male, A. Tuba Ozacar, Rita R. Fagan, Matthew Loring, Meng-Chieh Shen, Veronica Pace, Chris Devine, Alyssa Lutservitz, Rolf O. Karlstrom
bioRxiv 740613; doi: https://doi.org/10.1101/740613

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (1522)
  • Biochemistry (2475)
  • Bioengineering (1731)
  • Bioinformatics (9655)
  • Biophysics (3892)
  • Cancer Biology (2964)
  • Cell Biology (4185)
  • Clinical Trials (135)
  • Developmental Biology (2622)
  • Ecology (4092)
  • Epidemiology (2031)
  • Evolutionary Biology (6884)
  • Genetics (5202)
  • Genomics (6490)
  • Immunology (2181)
  • Microbiology (6928)
  • Molecular Biology (2750)
  • Neuroscience (17245)
  • Paleontology (126)
  • Pathology (425)
  • Pharmacology and Toxicology (705)
  • Physiology (1055)
  • Plant Biology (2484)
  • Scientific Communication and Education (642)
  • Synthetic Biology (828)
  • Systems Biology (2684)
  • Zoology (429)