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Bellymount enables longitudinal, intravital imaging of abdominal organs and the gut microbiota in adult Drosophila

Leslie Ann Jaramillo Koyama, View ORCID ProfileAndrés Aranda-Díaz, Yu-Han Su, Shruthi Balachandra, Judy Lisette Martin, William B. Ludington, Kerwyn Casey Huang, Lucy Erin O’Brien
doi: https://doi.org/10.1101/741991
Leslie Ann Jaramillo Koyama
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305
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Andrés Aranda-Díaz
Department of Bioengineering, Stanford University, Stanford, CA 94305
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  • ORCID record for Andrés Aranda-Díaz
Yu-Han Su
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305
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Shruthi Balachandra
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305
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Judy Lisette Martin
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305
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William B. Ludington
Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21218
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Kerwyn Casey Huang
Department of Bioengineering, Stanford University, Stanford, CA 94305Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305Chan Zuckerberg Biohub, San Francisco, CA 94158
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Lucy Erin O’Brien
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305
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  • For correspondence: lucye@stanford.edu
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Abstract

Cell- and tissue-level processes often occur across days or weeks, but few imaging methods can capture such long timescales. Here we describe Bellymount, a simple, non-invasive method for longitudinal imaging of the Drosophila abdomen at sub-cellular resolution. Bellymounted flies remain live and intact, so the same individual can be imaged serially to yield vivid time series of multi-day processes. This feature opens the door to longitudinal studies of Drosophila internal organs in their native context. Exploiting Bellymount’s capabilities, we track intestinal stem cell lineages and gut microbial colonization in single flies, revealing spatiotemporal dynamics undetectable by previously available methods.

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Posted August 21, 2019.
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Bellymount enables longitudinal, intravital imaging of abdominal organs and the gut microbiota in adult Drosophila
Leslie Ann Jaramillo Koyama, Andrés Aranda-Díaz, Yu-Han Su, Shruthi Balachandra, Judy Lisette Martin, William B. Ludington, Kerwyn Casey Huang, Lucy Erin O’Brien
bioRxiv 741991; doi: https://doi.org/10.1101/741991
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Bellymount enables longitudinal, intravital imaging of abdominal organs and the gut microbiota in adult Drosophila
Leslie Ann Jaramillo Koyama, Andrés Aranda-Díaz, Yu-Han Su, Shruthi Balachandra, Judy Lisette Martin, William B. Ludington, Kerwyn Casey Huang, Lucy Erin O’Brien
bioRxiv 741991; doi: https://doi.org/10.1101/741991

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