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Evoked and spontaneous pain assessment during tooth pulp injury

View ORCID ProfileHeather Lynn Rossi, Lily Pachanin See, William Foster, Saumitra Pitake, Jennifer Gibbs, Brian Schmidt, View ORCID ProfileClaire H. Mitchell, View ORCID ProfileIshmail Abdus-Saboor
doi: https://doi.org/10.1101/742049
Heather Lynn Rossi
Department of Biology, University of Pennsylvania, Philadelphia, PA
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Lily Pachanin See
Department of Endodontics, University of Pennsylvania, Philadelphia, PADepartment of Basic and Translational Science, University of Pennsylvania, Philadelphia, PA
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William Foster
Department of Biology, University of Pennsylvania, Philadelphia, PA
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Saumitra Pitake
Department of Biology, University of Pennsylvania, Philadelphia, PA
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Jennifer Gibbs
Department of Endodontics, Harvard University, Boston, MA
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Brian Schmidt
Department of Oral and Maxillofacial Surgery, New York University, New York, NY
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Claire H. Mitchell
Department of Basic and Translational Science, University of Pennsylvania, Philadelphia, PA
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Ishmail Abdus-Saboor
Department of Biology, University of Pennsylvania, Philadelphia, PA
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  • For correspondence: ishmail@sas.upenn.edu
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Abstract

Injury of the tooth pulp is excruciatingly painful and yet the receptors and neural circuit mechanisms that transmit this form of pain remain poorly defined in both the clinic and preclinical rodent models. Easily quantifiable behavioral assessment in the rodent orofacial area remains a major bottleneck in uncovering molecular mechanisms that govern inflammatory pain in the tooth. Here we use a dental pulp injury model in the mouse and expose the tooth pulp to the outside environment, a procedure we have previously shown produces pulpal inflammation. We demonstrate here with RNAscope technology in the trigeminal ganglion of injured mice, an upregulation of genes that contribute to the inflammatory pain state. Using both evoked and spontaneous measures of pain in the orofacial area, including application of von Frey Hair filaments and pain feature detection with the mouse grimace scale, we reveal a differential timeline of induction of spontaneous pain versus mechanical allodynia following pulpal injury. This work demonstrates that tooth pain can be easily assessed in freely behaving mice using approaches common for other types of pain assessment. Harnessing these assays in the orofacial area during gene manipulation should assist in uncovering mechanisms for tooth pulp inflammation and other forms of trigeminal pain.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 21, 2019.
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Evoked and spontaneous pain assessment during tooth pulp injury
Heather Lynn Rossi, Lily Pachanin See, William Foster, Saumitra Pitake, Jennifer Gibbs, Brian Schmidt, Claire H. Mitchell, Ishmail Abdus-Saboor
bioRxiv 742049; doi: https://doi.org/10.1101/742049
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Evoked and spontaneous pain assessment during tooth pulp injury
Heather Lynn Rossi, Lily Pachanin See, William Foster, Saumitra Pitake, Jennifer Gibbs, Brian Schmidt, Claire H. Mitchell, Ishmail Abdus-Saboor
bioRxiv 742049; doi: https://doi.org/10.1101/742049

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