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Autophagy mediates temporary reprogramming and dedifferentiation in plant somatic cells

Eleazar Rodriguez, Jonathan Chevalier, Jakob Olsen, Jeppe Ansbøl, Vaitsa Kapousidou, Zhangli Zuo, Steingrim Svenning, Christian Loefke, Stefanie Koemeda, Pedro Serrano Drozdowskyj, Jakub Jez, Gerhard Durnberger, Fabian Kuenzl, Michael Schutzbier, View ORCID ProfileKarl Mechtler, Signe Lolle, View ORCID ProfileYasin Dagdas, View ORCID ProfileMorten Petersen
doi: https://doi.org/10.1101/747410
Eleazar Rodriguez
Functional Genomic Section, Department of Biology, University of Copenhagen, 2220 Copenhagen, Denmark
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Jonathan Chevalier
Functional Genomic Section, Department of Biology, University of Copenhagen, 2220 Copenhagen, Denmark
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Jakob Olsen
Functional Genomic Section, Department of Biology, University of Copenhagen, 2220 Copenhagen, Denmark
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Jeppe Ansbøl
Functional Genomic Section, Department of Biology, University of Copenhagen, 2220 Copenhagen, Denmark
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Vaitsa Kapousidou
Functional Genomic Section, Department of Biology, University of Copenhagen, 2220 Copenhagen, Denmark
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Zhangli Zuo
Functional Genomic Section, Department of Biology, University of Copenhagen, 2220 Copenhagen, Denmark
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Steingrim Svenning
Molecular Cancer Research Group; Department of Medical Biology, University of Tromsø; Tromsø, Norway
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Christian Loefke
Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna BioCenter (VBC), Vienna
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Stefanie Koemeda
Vienna Biocenter Core Facilities (VBCF), Dr. Bohr-Gasse 3, 1030, Vienna, Austria.
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Pedro Serrano Drozdowskyj
Vienna Biocenter Core Facilities (VBCF), Dr. Bohr-Gasse 3, 1030, Vienna, Austria.
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Jakub Jez
Vienna Biocenter Core Facilities (VBCF), Dr. Bohr-Gasse 3, 1030, Vienna, Austria.
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Gerhard Durnberger
Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna BioCenter (VBC), ViennaVienna Biocenter Core Facilities (VBCF), Dr. Bohr-Gasse 3, 1030, Vienna, Austria.Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria
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Fabian Kuenzl
Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna BioCenter (VBC), Vienna
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Michael Schutzbier
Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna BioCenter (VBC), ViennaVienna Biocenter Core Facilities (VBCF), Dr. Bohr-Gasse 3, 1030, Vienna, Austria.Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria
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Karl Mechtler
Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna BioCenter (VBC), ViennaVienna Biocenter Core Facilities (VBCF), Dr. Bohr-Gasse 3, 1030, Vienna, Austria.Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria
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  • ORCID record for Karl Mechtler
Signe Lolle
Functional Genomic Section, Department of Biology, University of Copenhagen, 2220 Copenhagen, Denmark
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Yasin Dagdas
Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna BioCenter (VBC), Vienna
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  • ORCID record for Yasin Dagdas
  • For correspondence: shutko@bio.ku.dk
Morten Petersen
Functional Genomic Section, Department of Biology, University of Copenhagen, 2220 Copenhagen, Denmark
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  • ORCID record for Morten Petersen
  • For correspondence: shutko@bio.ku.dk
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Abstract

Somatic cells acclimate to changes in the environment by temporary reprogramming. Much has been learned about transcription factors that induce these cell-state switches in both plants and animals, but how cells rapidly modulate their proteome remains elusive. Here, we show rapid induction of autophagy during temporary reprogramming in plants triggered by phytohormones, immune and danger signals. Quantitative proteomics following sequential reprogramming revealed that autophagy is required for timely decay of previous cellular states and for tweaking the proteome to acclimate to the new conditions. Signatures of previous cellular programs thus persist in autophagy deficient cells, affecting cellular decision-making. Concordantly, autophagy deficient cells fail to acclimatize to dynamic climate changes. Similarly, they have defects in dedifferentiating into pluripotent stem cells, and redifferentiation during organogenesis. These observations indicate that autophagy mediates cell state switches that underlie somatic cell reprogramming in plants and possibly other organisms, and thereby promotes phenotypic plasticity.

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Posted August 28, 2019.
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Autophagy mediates temporary reprogramming and dedifferentiation in plant somatic cells
Eleazar Rodriguez, Jonathan Chevalier, Jakob Olsen, Jeppe Ansbøl, Vaitsa Kapousidou, Zhangli Zuo, Steingrim Svenning, Christian Loefke, Stefanie Koemeda, Pedro Serrano Drozdowskyj, Jakub Jez, Gerhard Durnberger, Fabian Kuenzl, Michael Schutzbier, Karl Mechtler, Signe Lolle, Yasin Dagdas, Morten Petersen
bioRxiv 747410; doi: https://doi.org/10.1101/747410
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Autophagy mediates temporary reprogramming and dedifferentiation in plant somatic cells
Eleazar Rodriguez, Jonathan Chevalier, Jakob Olsen, Jeppe Ansbøl, Vaitsa Kapousidou, Zhangli Zuo, Steingrim Svenning, Christian Loefke, Stefanie Koemeda, Pedro Serrano Drozdowskyj, Jakub Jez, Gerhard Durnberger, Fabian Kuenzl, Michael Schutzbier, Karl Mechtler, Signe Lolle, Yasin Dagdas, Morten Petersen
bioRxiv 747410; doi: https://doi.org/10.1101/747410

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