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Sag currents are a major contributor to human pyramidal cell intrinsic differences across cortical layers

Homeira Moradi Chameh, Scott Rich, Lihua Wang, Fu-Der Chen, Liang Zhang, Peter L. Carlen, Shreejoy J. Tripathy, Taufik A. Valiante
doi: https://doi.org/10.1101/748988
Homeira Moradi Chameh
1Krembil Research Institute, Division of Clinical and Computational Neuroscience
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Scott Rich
1Krembil Research Institute, Division of Clinical and Computational Neuroscience
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Lihua Wang
9Krembil Research Institute, Division of Experimental and Translational Neuroscience
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Fu-Der Chen
8Electrical and Computer Engineering, University of Toronto
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Liang Zhang
2Department of Physiology, University of Toronto
9Krembil Research Institute, Division of Experimental and Translational Neuroscience
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Peter L. Carlen
2Department of Physiology, University of Toronto
9Krembil Research Institute, Division of Experimental and Translational Neuroscience
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Shreejoy J. Tripathy
3Krembil Centre for Neuroinformatics, Centre for Addiction and Mental Health
4Department of Psychiatry, University of Toronto
5Institute of Medical Sciences, University of Toronto
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Taufik A. Valiante
1Krembil Research Institute, Division of Clinical and Computational Neuroscience
5Institute of Medical Sciences, University of Toronto
6Department of Surgery, Division of Neurosurgery, University of Toronto
7Institute of Biomaterials and Biomedical Engineering, University of Toronto
8Electrical and Computer Engineering, University of Toronto
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  • For correspondence: taufik.valiante@uhn.ca
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Abstract

In the human neocortex, coherent theta (∼8Hz) oscillations between superficial and deep cortical layers are driven by deep layer neurons, suggesting distinct intrinsic electrophysiological properties of L5 neurons. We used in vitro whole-cell recordings to characterize pyramidal cells in layer 2/3 (L2/3), layer 3c (L3c) and layer 5 (L5) of the human neocortex. L5 pyramidal cells were more excitable and had a more prominent sag relative to L2/3 and L3c neurons that was abolished by blockade of the hyperpolarization activated cation current (Ih). We found a greater proportion of L5 and L3c neurons displaying subthreshold resonance relative to L2/3. Although no theta subthreshold resonance was observed in either L5 and L2/3 neurons, L5 neurons were more adept at tracking both delta (4Hz) and theta oscillations, the former being dependent on Ih. The unique features of human L5 neurons likely contribute to the emergence of theta oscillations in human cortical microcircuits.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 25, 2020.
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Sag currents are a major contributor to human pyramidal cell intrinsic differences across cortical layers
Homeira Moradi Chameh, Scott Rich, Lihua Wang, Fu-Der Chen, Liang Zhang, Peter L. Carlen, Shreejoy J. Tripathy, Taufik A. Valiante
bioRxiv 748988; doi: https://doi.org/10.1101/748988
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Sag currents are a major contributor to human pyramidal cell intrinsic differences across cortical layers
Homeira Moradi Chameh, Scott Rich, Lihua Wang, Fu-Der Chen, Liang Zhang, Peter L. Carlen, Shreejoy J. Tripathy, Taufik A. Valiante
bioRxiv 748988; doi: https://doi.org/10.1101/748988

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