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ROP INTERACTIVE PARTNER b interacts with the ROP GTPase RACB and supports fungal penetration into barley epidermal cells

Christopher McCollum, Stefan Engelhardt, View ORCID ProfileRalph Hückelhoven
doi: https://doi.org/10.1101/750265
Christopher McCollum
Phytopathology, TUM School of Life Science Weihenstephan, Technical University of Munich, Emil-Ramann Str.2, 85354 Freising, Germany
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Stefan Engelhardt
Phytopathology, TUM School of Life Science Weihenstephan, Technical University of Munich, Emil-Ramann Str.2, 85354 Freising, Germany
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Ralph Hückelhoven
Phytopathology, TUM School of Life Science Weihenstephan, Technical University of Munich, Emil-Ramann Str.2, 85354 Freising, Germany
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  • ORCID record for Ralph Hückelhoven
  • For correspondence: hueckelhoven@wzw.tum.de
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Abstract

RHO of Plants (ROP) G-proteins are key components of cell polarization processes in plant development. The barley (Hordeum vulgare) ROP protein RACB, is a susceptibility factor in the interaction of barley with the barley powdery mildew fungus Blumeria graminis f.sp. hordei (Bgh). RACB also drives polar cell development, and this function might be coopted during formation of fungal haustoria in epidermal cells of barley. In order to understand RACB signaling during the interaction of barley with Bgh, we searched for potential downstream interactors of RACB. Here, we show that ROP INTERACTIVE PARTNER b (RIPb) directly interacts with RACB in yeast and in planta. Over-expression of RIPb supports susceptibility of barley to Bgh. RIPb further interacts with itself at microtubules. However, the interaction with activated RACB takes place at the plasma membrane. Both, RIPb and RACB are recruited to the site of fungal attack around the neck of developing haustoria suggesting locally enhanced ROP activity. We further assigned different functions to different domains of the RIPb protein. The N-terminal coiled-coil CC1 domain is required for microtubule localization, while the C-terminal coiled-coil CC2 domain is sufficient to interact with RACB and to fulfill a function in susceptibility at the plasma membrane. Hence, RIPb appears to locate at microtubules and is then recruited by activated RACB for a function at the plasma membrane during formation of the haustorial complex.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted August 29, 2019.
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ROP INTERACTIVE PARTNER b interacts with the ROP GTPase RACB and supports fungal penetration into barley epidermal cells
Christopher McCollum, Stefan Engelhardt, Ralph Hückelhoven
bioRxiv 750265; doi: https://doi.org/10.1101/750265
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ROP INTERACTIVE PARTNER b interacts with the ROP GTPase RACB and supports fungal penetration into barley epidermal cells
Christopher McCollum, Stefan Engelhardt, Ralph Hückelhoven
bioRxiv 750265; doi: https://doi.org/10.1101/750265

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