Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Securing the exchange of synthetic genetic constructs using digital signatures

View ORCID ProfileJenna E Gallegos, Diptendu M. Kar, View ORCID ProfileIndrakshi Ray, Indrajit Ray, View ORCID ProfileJean Peccoud
doi: https://doi.org/10.1101/750927
Jenna E Gallegos
1Colorado State University, Chemical and Biological Engineering
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jenna E Gallegos
Diptendu M. Kar
2Colorado State University, Computer Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Indrakshi Ray
2Colorado State University, Computer Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Indrakshi Ray
Indrajit Ray
2Colorado State University, Computer Sciences
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jean Peccoud
1Colorado State University, Chemical and Biological Engineering
3GenoFAB, Inc.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jean Peccoud
  • For correspondence: jean.peccoud@colostate.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Synthetic biology relies on an ever-growing supply chain of synthetic genetic material. Technologies to secure the exchange of this material are still in their infancy. Solutions proposed thus far have focused on watermarks, a dated security approach that can be used to claim authorship, but is subject to counterfeit, and does not provide any information about the integrity of the genetic material itself. We describe how data encryption and digital signature algorithms can be used to ensure the integrity and authenticity of synthetic genetic constructs. Using a pilot software that generates digital signatures and other encrypted data for plasmids, we demonstrate that we can predictably extract information about the author, the identity, and the integrity of plasmid sequences from sequencing data alone without a reference sequence, all without compromising the function of the plasmids. We discuss how this technology can be improved, applied, and expanded to support the new bioeconomy.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted September 26, 2019.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Securing the exchange of synthetic genetic constructs using digital signatures
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Securing the exchange of synthetic genetic constructs using digital signatures
Jenna E Gallegos, Diptendu M. Kar, Indrakshi Ray, Indrajit Ray, Jean Peccoud
bioRxiv 750927; doi: https://doi.org/10.1101/750927
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Securing the exchange of synthetic genetic constructs using digital signatures
Jenna E Gallegos, Diptendu M. Kar, Indrakshi Ray, Indrajit Ray, Jean Peccoud
bioRxiv 750927; doi: https://doi.org/10.1101/750927

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Synthetic Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (2441)
  • Biochemistry (4808)
  • Bioengineering (3342)
  • Bioinformatics (14730)
  • Biophysics (6665)
  • Cancer Biology (5194)
  • Cell Biology (7459)
  • Clinical Trials (138)
  • Developmental Biology (4388)
  • Ecology (6906)
  • Epidemiology (2057)
  • Evolutionary Biology (9948)
  • Genetics (7360)
  • Genomics (9555)
  • Immunology (4589)
  • Microbiology (12740)
  • Molecular Biology (4968)
  • Neuroscience (28450)
  • Paleontology (199)
  • Pathology (811)
  • Pharmacology and Toxicology (1400)
  • Physiology (2034)
  • Plant Biology (4528)
  • Scientific Communication and Education (981)
  • Synthetic Biology (1307)
  • Systems Biology (3923)
  • Zoology (731)