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Transient astrocytic Gq signaling underlies remote memory enhancement

Youichi Iwai, Katsuya Ozawa, Kazuko Yahagi, Mika Tanaka, Shigeyoshi Itohara, View ORCID ProfileHajime Hirase
doi: https://doi.org/10.1101/753095
Youichi Iwai
1Laboratory for Neuron-Glia Circuitry, RIKEN Center for Brain Science Wako, Saitama, Japan
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Katsuya Ozawa
1Laboratory for Neuron-Glia Circuitry, RIKEN Center for Brain Science Wako, Saitama, Japan
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Kazuko Yahagi
1Laboratory for Neuron-Glia Circuitry, RIKEN Center for Brain Science Wako, Saitama, Japan
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Mika Tanaka
1Laboratory for Neuron-Glia Circuitry, RIKEN Center for Brain Science Wako, Saitama, Japan
2Laboratory for Behavioral Genetics, RIKEN Center for Brain Science Wako, Saitama, Japan
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Shigeyoshi Itohara
2Laboratory for Behavioral Genetics, RIKEN Center for Brain Science Wako, Saitama, Japan
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Hajime Hirase
1Laboratory for Neuron-Glia Circuitry, RIKEN Center for Brain Science Wako, Saitama, Japan
3Center for Translational Neuromedicine Faculty of Medical and Health Sciences, University of Copenhagen, Copenhagen, Denmark
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  • ORCID record for Hajime Hirase
  • For correspondence: hirase@sund.ku.dk
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Abstract

Astrocytes elicit transient Ca2+ elevations induced by G protein-coupled receptors (GPCRs), yet their role in vivo remains unknown. To address this, transgenic mice with astrocytic expression of the optogenetic Gq-type GPCR, Optoα1AR, were established, in which transient Ca2+ elevations similar to those in wild type mice were induced by brief blue light illumination. Activation of cortical astrocytes resulted in an adenosine A1 receptor-dependent inhibition of neuronal activity. Moreover, sensory stimulation with astrocytic activation induced long-term depression of sensory evoked response. At the behavioral level, repeated astrocytic activation in the anterior cortex gradually affected novel open field exploratory behavior, and remote memory was enhanced in a novel object recognition task. These effects were blocked by A1 receptor antagonism. On the other hand, compelling evidence for astrocytic Ca2+-induced diameter changes of arteries was not observed. Together, we demonstrate that GPCR-triggered Ca2+ elevation in cortical astrocytes has causal impacts on neuronal activity and behavior.

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Posted September 02, 2019.
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Transient astrocytic Gq signaling underlies remote memory enhancement
Youichi Iwai, Katsuya Ozawa, Kazuko Yahagi, Mika Tanaka, Shigeyoshi Itohara, Hajime Hirase
bioRxiv 753095; doi: https://doi.org/10.1101/753095
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Transient astrocytic Gq signaling underlies remote memory enhancement
Youichi Iwai, Katsuya Ozawa, Kazuko Yahagi, Mika Tanaka, Shigeyoshi Itohara, Hajime Hirase
bioRxiv 753095; doi: https://doi.org/10.1101/753095

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