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A single-cell and single-nucleus RNA-seq toolbox for fresh and frozen human tumors
Michal Slyper, Caroline B. M. Porter, Orr Ashenberg, Julia Waldman, Eugene Drokhlyansky, Isaac Wakiro, Christopher Smillie, Gabriela Smith-Rosario, Jingyi Wu, Danielle Dionne, Sébastien Vigneau, Judit Jané-Valbuena, Sara Napolitano, Mei-Ju Su, Anand G. Patel, Asa Karlstrom, Simon Gritsch, Masashi Nomura, Avinash Waghray, Satyen H. Gohil, Alexander M. Tsankov, Livnat Jerby-Arnon, Ofir Cohen, Johanna Klughammer, Yanay Rosen, Joshua Gould, Bo Li, Lan Nguyen, Catherine J. Wu, Benjamin Izar, Rizwan Haq, F. Stephen Hodi, Charles H. Yoon, Aaron N. Hata, Suzanne J. Baker, Mario L. Suvà, Raphael Bueno, Elizabeth H. Stover, Ursula A. Matulonis, Michael R. Clay, Michael A. Dyer, Natalie B. Collins, Nikhil Wagle, Asaf Rotem, Bruce E. Johnson, Orit Rozenblatt-Rosen, Aviv Regev
doi: https://doi.org/10.1101/761429
Michal Slyper
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
Caroline B. M. Porter
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
Orr Ashenberg
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
Julia Waldman
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
Eugene Drokhlyansky
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
Isaac Wakiro
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
2Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA and Center for Cancer Precision Medicine of Dana-Farber Cancer Institute, Boston, MA 02215, USA
Christopher Smillie
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
Gabriela Smith-Rosario
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
Jingyi Wu
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
2Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA and Center for Cancer Precision Medicine of Dana-Farber Cancer Institute, Boston, MA 02215, USA
Danielle Dionne
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
Sébastien Vigneau
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
2Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA and Center for Cancer Precision Medicine of Dana-Farber Cancer Institute, Boston, MA 02215, USA
Judit Jané-Valbuena
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
Sara Napolitano
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
2Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA and Center for Cancer Precision Medicine of Dana-Farber Cancer Institute, Boston, MA 02215, USA
Mei-Ju Su
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
2Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA and Center for Cancer Precision Medicine of Dana-Farber Cancer Institute, Boston, MA 02215, USA
Anand G. Patel
3Department of Developmental Neurobiology, St. Jude Children’s Research Hospital, Memphis, TN 38105, USA
4Department of Oncology, St. Jude Children’s Research Hospital, Memphis, TN 38105, USA
Asa Karlstrom
3Department of Developmental Neurobiology, St. Jude Children’s Research Hospital, Memphis, TN 38105, USA
Simon Gritsch
5Department of Pathology and Center for Cancer Research, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Masashi Nomura
5Department of Pathology and Center for Cancer Research, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Avinash Waghray
6Massachusetts General Hospital, Harvard University, Boston, MA 02114, USA
Satyen H. Gohil
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
7Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA
Alexander M. Tsankov
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
Livnat Jerby-Arnon
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
Ofir Cohen
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
2Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA and Center for Cancer Precision Medicine of Dana-Farber Cancer Institute, Boston, MA 02215, USA
Johanna Klughammer
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
Yanay Rosen
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
Joshua Gould
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
Bo Li
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
8Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02129, USA
Lan Nguyen
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
Catherine J. Wu
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
7Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA
9Department of Internal Medicine, Brigham and Women’s Hospital, Boston, MA 02215, USA; Harvard Medical School, Boston, MA 02215, USA
Benjamin Izar
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
2Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA and Center for Cancer Precision Medicine of Dana-Farber Cancer Institute, Boston, MA 02215, USA
10Laboratory for Systems Pharmacology, Harvard Medical School, Boston, MA 02114, USA; Center for Immunology and Virology, Dana-Farber Cancer Institute, Boston, MA 02215, USA
11Ludwig Center for Cancer Research at Harvard, Boston, MA, USA
12Melanoma Disease Center, Dana-Farber Cancer Institute, Boston, MA 02215, USA
Rizwan Haq
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
7Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA
F. Stephen Hodi
7Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA
12Melanoma Disease Center, Dana-Farber Cancer Institute, Boston, MA 02215, USA
Charles H. Yoon
7Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA
13Brigham and Women’s Hospital, Department of Surgical Oncology, Boston, MA 02215, USA
Aaron N. Hata
14Massachusetts General Cancer Center, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Suzanne J. Baker
3Department of Developmental Neurobiology, St. Jude Children’s Research Hospital, Memphis, TN 38105, USA
Mario L. Suvà
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
5Department of Pathology and Center for Cancer Research, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Raphael Bueno
15Division of Thoracic Surgery, Brigham and Women’s Hospital, Boston, MA 02215, USA
Elizabeth H. Stover
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
7Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA
Ursula A. Matulonis
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
7Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA
Michael R. Clay
16Department of Pathology, St. Jude Children’s Research Hospital, Memphis, TN, 38105, USA
Michael A. Dyer
3Department of Developmental Neurobiology, St. Jude Children’s Research Hospital, Memphis, TN 38105, USA
17Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA
Natalie B. Collins
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
18Department of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA; Division of Pediatric Hematology and Oncology, Children’s Hospital, Boston, MA 02215, USA
Nikhil Wagle
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
2Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA and Center for Cancer Precision Medicine of Dana-Farber Cancer Institute, Boston, MA 02215, USA
Asaf Rotem
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
2Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA and Center for Cancer Precision Medicine of Dana-Farber Cancer Institute, Boston, MA 02215, USA
Bruce E. Johnson
2Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA and Center for Cancer Precision Medicine of Dana-Farber Cancer Institute, Boston, MA 02215, USA
Orit Rozenblatt-Rosen
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
Aviv Regev
1Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA
17Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA
19Koch Institute of Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Article usage
Posted September 12, 2019.
A single-cell and single-nucleus RNA-seq toolbox for fresh and frozen human tumors
Michal Slyper, Caroline B. M. Porter, Orr Ashenberg, Julia Waldman, Eugene Drokhlyansky, Isaac Wakiro, Christopher Smillie, Gabriela Smith-Rosario, Jingyi Wu, Danielle Dionne, Sébastien Vigneau, Judit Jané-Valbuena, Sara Napolitano, Mei-Ju Su, Anand G. Patel, Asa Karlstrom, Simon Gritsch, Masashi Nomura, Avinash Waghray, Satyen H. Gohil, Alexander M. Tsankov, Livnat Jerby-Arnon, Ofir Cohen, Johanna Klughammer, Yanay Rosen, Joshua Gould, Bo Li, Lan Nguyen, Catherine J. Wu, Benjamin Izar, Rizwan Haq, F. Stephen Hodi, Charles H. Yoon, Aaron N. Hata, Suzanne J. Baker, Mario L. Suvà, Raphael Bueno, Elizabeth H. Stover, Ursula A. Matulonis, Michael R. Clay, Michael A. Dyer, Natalie B. Collins, Nikhil Wagle, Asaf Rotem, Bruce E. Johnson, Orit Rozenblatt-Rosen, Aviv Regev
bioRxiv 761429; doi: https://doi.org/10.1101/761429
A single-cell and single-nucleus RNA-seq toolbox for fresh and frozen human tumors
Michal Slyper, Caroline B. M. Porter, Orr Ashenberg, Julia Waldman, Eugene Drokhlyansky, Isaac Wakiro, Christopher Smillie, Gabriela Smith-Rosario, Jingyi Wu, Danielle Dionne, Sébastien Vigneau, Judit Jané-Valbuena, Sara Napolitano, Mei-Ju Su, Anand G. Patel, Asa Karlstrom, Simon Gritsch, Masashi Nomura, Avinash Waghray, Satyen H. Gohil, Alexander M. Tsankov, Livnat Jerby-Arnon, Ofir Cohen, Johanna Klughammer, Yanay Rosen, Joshua Gould, Bo Li, Lan Nguyen, Catherine J. Wu, Benjamin Izar, Rizwan Haq, F. Stephen Hodi, Charles H. Yoon, Aaron N. Hata, Suzanne J. Baker, Mario L. Suvà, Raphael Bueno, Elizabeth H. Stover, Ursula A. Matulonis, Michael R. Clay, Michael A. Dyer, Natalie B. Collins, Nikhil Wagle, Asaf Rotem, Bruce E. Johnson, Orit Rozenblatt-Rosen, Aviv Regev
bioRxiv 761429; doi: https://doi.org/10.1101/761429
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