Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Delineating the rules for structural adaptation of membrane-associated proteins to evolutionary changes in membrane lipidome

Maria Makarova, Maria Peter, Gabor Balogh, Attila Glatz, James I. MacRae, Nestor Lopez Mora, Paula Booth, Eugene Makeyev, Laszlo Vigh, View ORCID ProfileSnezhana Oliferenko
doi: https://doi.org/10.1101/762146
Maria Makarova
1The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK
2Randall Centre for Cell and Molecular Biophysics, School of Basic and Medical Biosciences, King’s College London, London, SE1 1UL, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Maria Peter
3Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gabor Balogh
3Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Attila Glatz
3Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
James I. MacRae
1The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nestor Lopez Mora
4Department of Chemistry, King’s College London, Britannia House, 7 Trinity Street SE1 1DB, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Paula Booth
4Department of Chemistry, King’s College London, Britannia House, 7 Trinity Street SE1 1DB, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eugene Makeyev
5MRC Centre for Developmental Neurobiology, King’s College London, London, SE1 1UL, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Laszlo Vigh
3Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Snezhana Oliferenko
1The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK
2Randall Centre for Cell and Molecular Biophysics, School of Basic and Medical Biosciences, King’s College London, London, SE1 1UL, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Snezhana Oliferenko
  • For correspondence: snezhka.oliferenko@crick.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Summary

Membrane function is fundamental to life. Each species explores membrane lipid diversity within a genetically predefined range of possibilities. How membrane lipid composition in turn defines the functional space available for evolution of membrane-centered processes remains largely unknown. We address this fundamental question using related fission yeasts Schizosaccharomyces pombe and Schizosaccharomyces japonicus. We show that unlike S. pombe that generates membranes where both glycerophospholipid acyl tails are predominantly 16-18 carbons long, S. japonicus synthesizes unusual ‘asymmetrical’ glycerophospholipids where the tails differ in length by 6-8 carbons. This results in stiffer bilayers with distinct lipid packing properties. Retroengineered S. pombe synthesizing the S. japonicus-type phospholipids exhibits unfolded protein response and downregulates secretion. Importantly, our protein sequence comparisons and domain swap experiments indicate that transmembrane helices co-evolve with membranes, suggesting that, on the evolutionary scale, changes in membrane lipid composition may necessitate extensive adaptation of the membrane-associated proteome.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted September 09, 2019.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Delineating the rules for structural adaptation of membrane-associated proteins to evolutionary changes in membrane lipidome
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Delineating the rules for structural adaptation of membrane-associated proteins to evolutionary changes in membrane lipidome
Maria Makarova, Maria Peter, Gabor Balogh, Attila Glatz, James I. MacRae, Nestor Lopez Mora, Paula Booth, Eugene Makeyev, Laszlo Vigh, Snezhana Oliferenko
bioRxiv 762146; doi: https://doi.org/10.1101/762146
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Delineating the rules for structural adaptation of membrane-associated proteins to evolutionary changes in membrane lipidome
Maria Makarova, Maria Peter, Gabor Balogh, Attila Glatz, James I. MacRae, Nestor Lopez Mora, Paula Booth, Eugene Makeyev, Laszlo Vigh, Snezhana Oliferenko
bioRxiv 762146; doi: https://doi.org/10.1101/762146

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3691)
  • Biochemistry (7800)
  • Bioengineering (5678)
  • Bioinformatics (21295)
  • Biophysics (10584)
  • Cancer Biology (8179)
  • Cell Biology (11948)
  • Clinical Trials (138)
  • Developmental Biology (6764)
  • Ecology (10401)
  • Epidemiology (2065)
  • Evolutionary Biology (13876)
  • Genetics (9709)
  • Genomics (13075)
  • Immunology (8151)
  • Microbiology (20022)
  • Molecular Biology (7859)
  • Neuroscience (43073)
  • Paleontology (321)
  • Pathology (1279)
  • Pharmacology and Toxicology (2261)
  • Physiology (3353)
  • Plant Biology (7232)
  • Scientific Communication and Education (1314)
  • Synthetic Biology (2008)
  • Systems Biology (5539)
  • Zoology (1128)