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Primary cilium-dependent cAMP/PKA signaling at the centrosome regulates neuronal migration

Julie Stoufflet, Maxime Chaulet, Mohamed Doulazmi, Coralie Fouquet, Caroline Dubacq, Christine Métin, Alain Trembleau, Pierre Vincent, Isabelle Caillé
doi: https://doi.org/10.1101/765925
Julie Stoufflet
1Sorbonne Université, CNRS UMR8246, Inserm U1130, Institut de Biologie Paris Seine (IBPS), Neuroscience Paris Seine (NPS), F-75005 Paris, France
2Sorbonne Université, CNRS UMR8256, Institut Biologie Paris Seine (IBPS), Biological adaptation and ageing (B2A), F-75005 Paris, France
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Maxime Chaulet
1Sorbonne Université, CNRS UMR8246, Inserm U1130, Institut de Biologie Paris Seine (IBPS), Neuroscience Paris Seine (NPS), F-75005 Paris, France
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Mohamed Doulazmi
2Sorbonne Université, CNRS UMR8256, Institut Biologie Paris Seine (IBPS), Biological adaptation and ageing (B2A), F-75005 Paris, France
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Coralie Fouquet
1Sorbonne Université, CNRS UMR8246, Inserm U1130, Institut de Biologie Paris Seine (IBPS), Neuroscience Paris Seine (NPS), F-75005 Paris, France
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Caroline Dubacq
1Sorbonne Université, CNRS UMR8246, Inserm U1130, Institut de Biologie Paris Seine (IBPS), Neuroscience Paris Seine (NPS), F-75005 Paris, France
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Christine Métin
3Inserm UMR-S839. Institut du Fer à Moulin, Sorbonne Université, Paris, France
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Alain Trembleau
1Sorbonne Université, CNRS UMR8246, Inserm U1130, Institut de Biologie Paris Seine (IBPS), Neuroscience Paris Seine (NPS), F-75005 Paris, France
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Pierre Vincent
2Sorbonne Université, CNRS UMR8256, Institut Biologie Paris Seine (IBPS), Biological adaptation and ageing (B2A), F-75005 Paris, France
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  • For correspondence: isabelle.caille@upmc.fr pierre.vincent@upmc.fr
Isabelle Caillé
1Sorbonne Université, CNRS UMR8246, Inserm U1130, Institut de Biologie Paris Seine (IBPS), Neuroscience Paris Seine (NPS), F-75005 Paris, France
4Université de Paris, Paris, France
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  • For correspondence: isabelle.caille@upmc.fr pierre.vincent@upmc.fr
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  • Movie 1: cAMP biosensor in control migrating neuron[supplements/765925_file02.avi]
  • Movie 2: cAMP insensitive biosensor in control migrating neuron[supplements/765925_file03.avi]
  • Movie 3: cAMP biosensor in Kif3a recombined migrating neuron[supplements/765925_file04.avi]
  • Movie 4: cAMP biosensor in RPGrip1L recombined migrating neuron[supplements/765925_file05.avi]
  • Movie 5: cAMP biosensor in AC3 knocked-down migrating neuron[supplements/765925_file06.avi]
  • Movie 6: GFP migrating neurons (control condition)[supplements/765925_file07.avi]
  • Movie 7: Cre-GFP migrating neurons (Kif3a recombined condition)[supplements/765925_file08.avi]
  • Movie 8: MirAC3-GFP migrating neurons (AC3 KD condition)[supplements/765925_file09.avi]
  • Movie 9: GFP + CentrinRFP migrating neuron (control condition)[supplements/765925_file10.avi]
  • Movie 10: Cre-GFP + CentrinRFP migrating neuron (Kif3a recombined condition)[supplements/765925_file11.avi]
  • Movie 11: dnPKA-GFP + tdTomato migrating neurons (delocalized PKA condition)[supplements/765925_file12.avi]
  • Movie 12: dnPKA-GFP + Cre-tdTomato migrating neurons (delocalized PKA + Kif3a recombined condition)[supplements/765925_file13.avi]
  • Movie 13: cAMP biosensor in radially migrating neuron of the postnatal olfactory bulb[supplements/765925_file14.avi]
  • Movie 14: cAMP biosensor in tangentially migrating neuron of the adult RMS[supplements/765925_file15.avi]
  • Movie 15: cAMP biosensor in radially migrating neuron of the embryonic cortex (E19)[supplements/765925_file16.avi]
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Posted November 12, 2019.
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Primary cilium-dependent cAMP/PKA signaling at the centrosome regulates neuronal migration
Julie Stoufflet, Maxime Chaulet, Mohamed Doulazmi, Coralie Fouquet, Caroline Dubacq, Christine Métin, Alain Trembleau, Pierre Vincent, Isabelle Caillé
bioRxiv 765925; doi: https://doi.org/10.1101/765925
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Primary cilium-dependent cAMP/PKA signaling at the centrosome regulates neuronal migration
Julie Stoufflet, Maxime Chaulet, Mohamed Doulazmi, Coralie Fouquet, Caroline Dubacq, Christine Métin, Alain Trembleau, Pierre Vincent, Isabelle Caillé
bioRxiv 765925; doi: https://doi.org/10.1101/765925

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