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Inosine is an alternative carbon supply that supports effector T cell proliferation and antitumor function under glucose restriction

Tingting Wang, JN Rashida Gnanaprakasam, Xuyong Chen, Siwen Kang, Xuequn Xu, Hua Sun, Lingling Liu, Ethan Miller, Teresa A. Cassel, Qiushi Sun, Sara Vicente-Muñoz, Marc O. Warmoes, Andrew N. Lane, Xiaotong Song, Teresa W.-M. Fan, Ruoning Wang
doi: https://doi.org/10.1101/766642
Tingting Wang
1Center for Childhood Cancer & Blood Diseases, Hematology/Oncology & BMT, Abigail Wexner Research Institute at Nationwide Children’s Hospital, The Ohio State University, Columbus, OH, USA
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JN Rashida Gnanaprakasam
1Center for Childhood Cancer & Blood Diseases, Hematology/Oncology & BMT, Abigail Wexner Research Institute at Nationwide Children’s Hospital, The Ohio State University, Columbus, OH, USA
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Xuyong Chen
1Center for Childhood Cancer & Blood Diseases, Hematology/Oncology & BMT, Abigail Wexner Research Institute at Nationwide Children’s Hospital, The Ohio State University, Columbus, OH, USA
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Siwen Kang
1Center for Childhood Cancer & Blood Diseases, Hematology/Oncology & BMT, Abigail Wexner Research Institute at Nationwide Children’s Hospital, The Ohio State University, Columbus, OH, USA
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Xuequn Xu
1Center for Childhood Cancer & Blood Diseases, Hematology/Oncology & BMT, Abigail Wexner Research Institute at Nationwide Children’s Hospital, The Ohio State University, Columbus, OH, USA
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Hua Sun
3The Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030 USA
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Lingling Liu
1Center for Childhood Cancer & Blood Diseases, Hematology/Oncology & BMT, Abigail Wexner Research Institute at Nationwide Children’s Hospital, The Ohio State University, Columbus, OH, USA
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Ethan Miller
1Center for Childhood Cancer & Blood Diseases, Hematology/Oncology & BMT, Abigail Wexner Research Institute at Nationwide Children’s Hospital, The Ohio State University, Columbus, OH, USA
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Teresa A. Cassel
2Center for Environmental and Systems Biochemistry, Dept. of Toxicology and Cancer Biology, Markey Cancer Center, University of Kentucky, Lexington, KY 40536, USA
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Qiushi Sun
2Center for Environmental and Systems Biochemistry, Dept. of Toxicology and Cancer Biology, Markey Cancer Center, University of Kentucky, Lexington, KY 40536, USA
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Sara Vicente-Muñoz
2Center for Environmental and Systems Biochemistry, Dept. of Toxicology and Cancer Biology, Markey Cancer Center, University of Kentucky, Lexington, KY 40536, USA
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Marc O. Warmoes
2Center for Environmental and Systems Biochemistry, Dept. of Toxicology and Cancer Biology, Markey Cancer Center, University of Kentucky, Lexington, KY 40536, USA
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Andrew N. Lane
2Center for Environmental and Systems Biochemistry, Dept. of Toxicology and Cancer Biology, Markey Cancer Center, University of Kentucky, Lexington, KY 40536, USA
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Xiaotong Song
3The Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030 USA
4Icell Kealex Therapeutics, Houston, TX 77021
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  • For correspondence: ruoning.wang@nationwidechildrens.org twmfan@gmail.com shautong.song@icellkealex.com
Teresa W.-M. Fan
2Center for Environmental and Systems Biochemistry, Dept. of Toxicology and Cancer Biology, Markey Cancer Center, University of Kentucky, Lexington, KY 40536, USA
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  • For correspondence: ruoning.wang@nationwidechildrens.org twmfan@gmail.com shautong.song@icellkealex.com
Ruoning Wang
1Center for Childhood Cancer & Blood Diseases, Hematology/Oncology & BMT, Abigail Wexner Research Institute at Nationwide Children’s Hospital, The Ohio State University, Columbus, OH, USA
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  • For correspondence: ruoning.wang@nationwidechildrens.org twmfan@gmail.com shautong.song@icellkealex.com
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Article Information

doi 
https://doi.org/10.1101/766642
History 
  • September 14, 2019.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.

Author Information

  1. Tingting Wang1,*,
  2. JN Rashida Gnanaprakasam1,*,
  3. Xuyong Chen1,
  4. Siwen Kang1,
  5. Xuequn Xu1,
  6. Hua Sun3,
  7. Lingling Liu1,
  8. Ethan Miller1,
  9. Teresa A. Cassel2,
  10. Qiushi Sun2,
  11. Sara Vicente-Muñoz2,
  12. Marc O. Warmoes2,
  13. Andrew N. Lane2,
  14. Xiaotong Song3,4,$,
  15. Teresa W.-M. Fan2,$ and
  16. Ruoning Wang1,$
  1. 1Center for Childhood Cancer & Blood Diseases, Hematology/Oncology & BMT, Abigail Wexner Research Institute at Nationwide Children’s Hospital, The Ohio State University, Columbus, OH, USA
  2. 2Center for Environmental and Systems Biochemistry, Dept. of Toxicology and Cancer Biology, Markey Cancer Center, University of Kentucky, Lexington, KY 40536, USA
  3. 3The Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030 USA
  4. 4Icell Kealex Therapeutics, Houston, TX 77021
  1. ↵$Correspondence should be addressed to: Ruoning Wang, Phone: 614-335-2980; Fax: 614-722-5895. ruoning.wang{at}nationwidechildrens.org; Teresa W.-M. Fan, Phone: 858-218-1028, Fax: 859-257-1307, twmfan{at}gmail.com; Xiaotong Song; shautong.song{at}icellkealex.com;
  1. ↵* Wang T and Gnanaprakasam J contributed equally to this paper

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Inosine is an alternative carbon supply that supports effector T cell proliferation and antitumor function under glucose restriction
Tingting Wang, JN Rashida Gnanaprakasam, Xuyong Chen, Siwen Kang, Xuequn Xu, Hua Sun, Lingling Liu, Ethan Miller, Teresa A. Cassel, Qiushi Sun, Sara Vicente-Muñoz, Marc O. Warmoes, Andrew N. Lane, Xiaotong Song, Teresa W.-M. Fan, Ruoning Wang
bioRxiv 766642; doi: https://doi.org/10.1101/766642
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Inosine is an alternative carbon supply that supports effector T cell proliferation and antitumor function under glucose restriction
Tingting Wang, JN Rashida Gnanaprakasam, Xuyong Chen, Siwen Kang, Xuequn Xu, Hua Sun, Lingling Liu, Ethan Miller, Teresa A. Cassel, Qiushi Sun, Sara Vicente-Muñoz, Marc O. Warmoes, Andrew N. Lane, Xiaotong Song, Teresa W.-M. Fan, Ruoning Wang
bioRxiv 766642; doi: https://doi.org/10.1101/766642

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