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Arabidopsis PAD4 lipase-like domain is a minimal functional unit in resistance to green peach aphid

View ORCID ProfileJoram A. Dongus, View ORCID ProfileDeepak D. Bhandari, View ORCID ProfileMonika Patel, View ORCID ProfileLani Archer, View ORCID ProfileLucas Dijkgraaf, View ORCID ProfileLaurent Deslandes, View ORCID ProfileJyoti Shah, View ORCID ProfileJane E. Parker
doi: https://doi.org/10.1101/769125
Joram A. Dongus
1Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-weg 10; 50829 Cologne, Germany
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Deepak D. Bhandari
1Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-weg 10; 50829 Cologne, Germany
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Monika Patel
2Department of Biological Sciences, University of North Texas; 1511 West Sycamore, Denton, Texas 76201, U.S.A.
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Lani Archer
2Department of Biological Sciences, University of North Texas; 1511 West Sycamore, Denton, Texas 76201, U.S.A.
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Lucas Dijkgraaf
1Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-weg 10; 50829 Cologne, Germany
3Plant-Microbe Interactions - Utrecht University, Padualaan 8; 3584 CH Utrecht, Netherlands
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Laurent Deslandes
4LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France
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Jyoti Shah
2Department of Biological Sciences, University of North Texas; 1511 West Sycamore, Denton, Texas 76201, U.S.A.
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Jane E. Parker
1Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-weg 10; 50829 Cologne, Germany
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  • For correspondence: parker@mpipz.mpg.de
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Abstract

Plants have evolved mechanisms to attract beneficial microbes and insects while protecting themselves against pathogenic microbes and pests. In Arabidopsis, the immune regulator PAD4 functions with its cognate partner EDS1 to limit pathogen growth. PAD4, independently of EDS1, reduces infestation by Green Peach Aphid (GPA). How PAD4 regulates these defense outputs is unclear. By expressing the N-terminal PAD4-lipase-like domain (LLD) without its C-terminal ‘EDS1-PAD4’ (EP) domain, we interrogated PAD4 functions in plant defense. Here we show that transgenic expression of PAD4LLD in Arabidopsis is sufficient for limiting GPA infestation, but not for conferring basal and effector-triggered pathogen immunity. This suggests that the C-terminal PAD4-EP domain is necessary for EDS1-dependent immune functions. Moreover, PAD4LLD is not sufficient to interact with EDS1, indicating the PAD4-EP domain is required for heterodimerisation. These data provide molecular evidence that PAD4 has domain specific functions.

Footnotes

  • Funding: This work was supported by the Max Planck Society and Deutsche Forschungsgemeinschaft (DFG) Grants: DFG-ANR Trilateral ‘RADAR’ grant (JAD, LaD (ANR-15-CE20-0016-01), JEP), CRC670 (DDB, JEP), and MP was supported by a scholarship from the University of North Texas.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted September 14, 2019.
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Arabidopsis PAD4 lipase-like domain is a minimal functional unit in resistance to green peach aphid
Joram A. Dongus, Deepak D. Bhandari, Monika Patel, Lani Archer, Lucas Dijkgraaf, Laurent Deslandes, Jyoti Shah, Jane E. Parker
bioRxiv 769125; doi: https://doi.org/10.1101/769125
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Arabidopsis PAD4 lipase-like domain is a minimal functional unit in resistance to green peach aphid
Joram A. Dongus, Deepak D. Bhandari, Monika Patel, Lani Archer, Lucas Dijkgraaf, Laurent Deslandes, Jyoti Shah, Jane E. Parker
bioRxiv 769125; doi: https://doi.org/10.1101/769125

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