Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Performance of Turquoise killifish, model organism in aging, on commercial pelleted diet: a step towards husbandry standardization

View ORCID ProfileJakub Žák, View ORCID ProfileIva Dyková, View ORCID ProfileMarin Reichard
doi: https://doi.org/10.1101/770479
Jakub Žák
Czech Academy of Sciences, Institute of Vertebrate Biology, Květná 8, 603 65, Brno, Czech RepublicDepartment of Zoology, Faculty of Sciences, Charles University, Viničná 7, 122 44, Prague, Czech Republic
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jakub Žák
Iva Dyková
Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, Brno, 611 37 Czech Republic
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Iva Dyková
Marin Reichard
Czech Academy of Sciences, Institute of Vertebrate Biology, Květná 8, 603 65, Brno, Czech Republic
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Marin Reichard
  • For correspondence: reichard@ivb.cz
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

SUMMARY

Dietary alteration is one of the most universally effective aging interventions, making its standardization a fundamental need for model organisms in aging. Here we address the current lack of standardized formulated diet for Turquoise Killifish Nothobranchius furzeri – a promising model organism. We first demonstrated that N. furzeri can be fully weaned onto a standardized commercially available pelleted diet as the sole nutrition when kept in social tanks. We then compared nine somatic and six reproductive parameters between fish fed a typical laboratory diet - frozen chironomid larvae (bloodworms) and fish fed solely on BioMar pellets. Killifish readily consumed the pellets. Although fish consumed 7.5 times less food mass in the form of pellets than bloodworms, they had comparable somatic and reproductive performance. There was no difference between diet groups in body size, specific growth rate, condition or extent of hepatocellular vacuolation. Fish fed a pelleted diet had higher juvenile body mass and more visceral fat. Pellet-fed males had lower liver mass and possessed a lipid type of hepatocellular vacuolation instead of the prevailing glycogen-like vacuolation in the bloodworm-fed group. No significant effect was found on reproductive parameters. The negligible differences between dietary groups and good acceptance of pellets indicates their suitability as a useful starting point for diet standardization (and potential manipulation) in Nothobranchius furzeri.

Footnotes

  • Jakub Žák: jakub.zak{at}natur.cuni.cz, Iva Dyková: dykova.iva{at}gmail.com

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted September 16, 2019.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Performance of Turquoise killifish, model organism in aging, on commercial pelleted diet: a step towards husbandry standardization
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
Share
Performance of Turquoise killifish, model organism in aging, on commercial pelleted diet: a step towards husbandry standardization
Jakub Žák, Iva Dyková, Marin Reichard
bioRxiv 770479; doi: https://doi.org/10.1101/770479
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Performance of Turquoise killifish, model organism in aging, on commercial pelleted diet: a step towards husbandry standardization
Jakub Žák, Iva Dyková, Marin Reichard
bioRxiv 770479; doi: https://doi.org/10.1101/770479

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Physiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (1647)
  • Biochemistry (2739)
  • Bioengineering (1907)
  • Bioinformatics (10243)
  • Biophysics (4183)
  • Cancer Biology (3218)
  • Cell Biology (4539)
  • Clinical Trials (135)
  • Developmental Biology (2840)
  • Ecology (4461)
  • Epidemiology (2041)
  • Evolutionary Biology (7231)
  • Genetics (5477)
  • Genomics (6813)
  • Immunology (2390)
  • Microbiology (7485)
  • Molecular Biology (2993)
  • Neuroscience (18584)
  • Paleontology (136)
  • Pathology (472)
  • Pharmacology and Toxicology (780)
  • Physiology (1150)
  • Plant Biology (2706)
  • Scientific Communication and Education (680)
  • Synthetic Biology (888)
  • Systems Biology (2846)
  • Zoology (468)