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A deeper understanding of intestinal organoid metabolism revealed by combining fluorescence lifetime imaging microscopy (FLIM) and extracellular flux analyses

View ORCID ProfileIrina A. Okkelman, Nuno Neto, View ORCID ProfileDmitri B. Papkovsky, View ORCID ProfileMichael Monaghan, View ORCID ProfileRuslan I. Dmitriev
doi: https://doi.org/10.1101/771188
Irina A. Okkelman
1Laboratory of Biophysics and Bioanalysis, ABCRF, University College Cork, Cavanagh Pharmacy Building, College Road, Cork, T12 K8AF, Ireland
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Nuno Neto
2Department of Mechanical and Manufacturing Engineering, Trinity College Dublin, Ireland
3Trinity Centre for Biomedical Engineering, Trinity College Dublin, Ireland
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Dmitri B. Papkovsky
1Laboratory of Biophysics and Bioanalysis, ABCRF, University College Cork, Cavanagh Pharmacy Building, College Road, Cork, T12 K8AF, Ireland
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Michael Monaghan
2Department of Mechanical and Manufacturing Engineering, Trinity College Dublin, Ireland
3Trinity Centre for Biomedical Engineering, Trinity College Dublin, Ireland
4Advanced Materials and BioEngineering Research (AMBER) Centre at Trinity College Dublin and Royal College of Surgeons in Ireland, Dublin, Ireland
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Ruslan I. Dmitriev
1Laboratory of Biophysics and Bioanalysis, ABCRF, University College Cork, Cavanagh Pharmacy Building, College Road, Cork, T12 K8AF, Ireland
5Institute for Regenerative Medicine, I.M. Sechenov First Moscow State University, Moscow, Russian Federation
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  • For correspondence: r.dmitriev@ucc.ie
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Abstract

Stem cells and the niche in which they reside feature a complex microenvironment with tightly regulated homeostasis, cell-cell interactions and dynamic regulation of metabolism. A significant number of organoid models has been described over the last decade, yet few methodologies can enable single cell level resolution analysis of the stem cell niche metabolic demands, in real-time and without perturbing integrity. Here, we studied the redox metabolism of Lgr5-GFP intestinal organoids by two emerging microscopy approaches based on luminescence lifetime measurement – fluorescence-based FLIM for NAD(P)H, and phosphorescence-based PLIM for real-time oxygenation. We found that exposure of stem (Lgr5-GFP) and differentiated (no GFP) cells to high and low glucose concentrations resulted in measurable shifts in oxygenation and redox status. NAD(P)H-FLIM and O2-PLIM both indicated that at high ‘basal’ glucose conditions, Lgr5-GFP cells had lower activity of oxidative phosphorylation when compared with cells lacking Lgr5. However, when exposed to low (0.5 mM) glucose, stem cells utilized oxidative metabolism more dynamically than non-stem cells. The high heterogeneity of complex 3D architecture and energy production pathways of Lgr5-GFP organoids were also confirmed by the extracellular flux (XF) analysis. Our data reveals that combined analysis of NAD(P)H-FLIM and organoid oxygenation by PLIM represents promising approach for studying stem cell niche metabolism in a live readout.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted September 16, 2019.
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A deeper understanding of intestinal organoid metabolism revealed by combining fluorescence lifetime imaging microscopy (FLIM) and extracellular flux analyses
Irina A. Okkelman, Nuno Neto, Dmitri B. Papkovsky, Michael Monaghan, Ruslan I. Dmitriev
bioRxiv 771188; doi: https://doi.org/10.1101/771188
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A deeper understanding of intestinal organoid metabolism revealed by combining fluorescence lifetime imaging microscopy (FLIM) and extracellular flux analyses
Irina A. Okkelman, Nuno Neto, Dmitri B. Papkovsky, Michael Monaghan, Ruslan I. Dmitriev
bioRxiv 771188; doi: https://doi.org/10.1101/771188

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