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Correlative three-dimensional super-resolution and block face electron microscopy of whole vitreously frozen cells
View ORCID ProfileDavid P. Hoffman, Gleb Shtengel, View ORCID ProfileC. Shan Xu, View ORCID ProfileKirby R. Campbell, Melanie Freeman, Lei Wang, View ORCID ProfileDaniel E. Milkie, H. Amalia Pasolli, Nirmala Iyer, View ORCID ProfileJohn A. Bogovic, Daniel R. Stabley, View ORCID ProfileAbbas Shirinifard, Song Pang, David Peale, Kathy Schaefer, View ORCID ProfileWim Pomp, View ORCID ProfileChi-Lun Chang, View ORCID ProfileJennifer Lippincott-Schwartz, View ORCID ProfileTom Kirchhausen, View ORCID ProfileDavid J. Solecki, View ORCID ProfileEric Betzig, View ORCID ProfileHarald Hess
doi: https://doi.org/10.1101/773986
David P. Hoffman
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
Gleb Shtengel
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
C. Shan Xu
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
Kirby R. Campbell
2Department of Developmental Neurobiology, St. Jude Children’s Research Hospital, 262 Danny Thomas Pl, Memphis, TN 38105 USA
Melanie Freeman
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
Lei Wang
3Department of Cell Biology, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115, USA
4Program in Cellular and Molecular Medicine, Boston Children’s Hospital, 200 Longwood Ave, Boston, MA 02115, USA
5Department of Pediatrics, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115, USA
Daniel E. Milkie
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
H. Amalia Pasolli
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
Nirmala Iyer
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
John A. Bogovic
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
Daniel R. Stabley
6Neuroimaging Laboratory, St. Jude Children’s Research Hospital, 262 Danny Thomas Pl, Memphis, TN 38105 USA
Abbas Shirinifard
7Bioimage Analysis Core, St. Jude Children’s Research Hospital, 262 Danny Thomas Pl, Memphis, TN 38105 USA
Song Pang
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
David Peale
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
Kathy Schaefer
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
Wim Pomp
3Department of Cell Biology, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115, USA
4Program in Cellular and Molecular Medicine, Boston Children’s Hospital, 200 Longwood Ave, Boston, MA 02115, USA
5Department of Pediatrics, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115, USA
Chi-Lun Chang
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
Jennifer Lippincott-Schwartz
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
Tom Kirchhausen
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
3Department of Cell Biology, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115, USA
4Program in Cellular and Molecular Medicine, Boston Children’s Hospital, 200 Longwood Ave, Boston, MA 02115, USA
5Department of Pediatrics, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115, USA
David J. Solecki
2Department of Developmental Neurobiology, St. Jude Children’s Research Hospital, 262 Danny Thomas Pl, Memphis, TN 38105 USA
Eric Betzig
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
8Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
9Department of Physics, University of California, Berkeley, CA 94720, USA
10Howard Hughes Medical Institute, Berkeley, CA 94720, USA
11Helen Wills Neuroscience Institute, Berkeley, CA 94720, USA
12Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
Harald Hess
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
Article usage
Posted September 18, 2019.
Correlative three-dimensional super-resolution and block face electron microscopy of whole vitreously frozen cells
David P. Hoffman, Gleb Shtengel, C. Shan Xu, Kirby R. Campbell, Melanie Freeman, Lei Wang, Daniel E. Milkie, H. Amalia Pasolli, Nirmala Iyer, John A. Bogovic, Daniel R. Stabley, Abbas Shirinifard, Song Pang, David Peale, Kathy Schaefer, Wim Pomp, Chi-Lun Chang, Jennifer Lippincott-Schwartz, Tom Kirchhausen, David J. Solecki, Eric Betzig, Harald Hess
bioRxiv 773986; doi: https://doi.org/10.1101/773986
Correlative three-dimensional super-resolution and block face electron microscopy of whole vitreously frozen cells
David P. Hoffman, Gleb Shtengel, C. Shan Xu, Kirby R. Campbell, Melanie Freeman, Lei Wang, Daniel E. Milkie, H. Amalia Pasolli, Nirmala Iyer, John A. Bogovic, Daniel R. Stabley, Abbas Shirinifard, Song Pang, David Peale, Kathy Schaefer, Wim Pomp, Chi-Lun Chang, Jennifer Lippincott-Schwartz, Tom Kirchhausen, David J. Solecki, Eric Betzig, Harald Hess
bioRxiv 773986; doi: https://doi.org/10.1101/773986
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