Abstract
A well coordinated neuro-olfactory regulation is essential for a successful host seeking and blood feeding behavior of adult female mosquitoes. Although, the crucial role of mosquito tiny brain during host selection/choice is of prime interest but how blood meal uptake modulate brain functions which consequently empower the cognition in mosquitoes remains unclear. Here we report that blood meal induced gut ‘metabolic switch’ shift brain function from external communication to inter-organ management. An exclusive induction of oxidation reduction associated proteins and enhancement of brain’s energy metabolism indicate the active functioning of the brain probably to maintain physiological homeostasis. A spatial and temporal change in the expression pattern of neuro-signaling molecules and other neuro-modulators in the brain designate that a guided neuro-stimulation and endocrine regulation is enough to manage the brain-distant organ communication. Analogous to brain, significant modulation of the neuro-modulator receptor genes in the gut signifies its function as ‘Second Brain’ during metabolic switch in mosquitoes. Furthermore, quantitative analysis of neuro-transmitters (NTs)revealed that the gut of mosquitoes is one of the potent source of NTs during altered metabolic conditions. Finally, quantitative data on NTs dynamics of naïve and aseptic mosquitoes establish the concept of microbiome-gut-brain-axis communication in adult female mosquitoes.