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Single-cell atlas of major haematopoietic tissues sheds light on blood cell formation from embryonic endothelium

View ORCID ProfileMaya Shvartsman, View ORCID ProfilePolina V. Pavlovich, View ORCID ProfileMorgan Oatley, View ORCID ProfileKerstin Ganter, View ORCID ProfileRachel McKernan, View ORCID ProfileRadvile Prialgauskaite, View ORCID ProfileArtem Adamov, Konstantin Chukreev, Nicolas Descostes, View ORCID ProfileAndreas Buness, View ORCID ProfileNina Cabezas-Wallscheid, View ORCID ProfileChristophe Lancrin
doi: https://doi.org/10.1101/774547
Maya Shvartsman
1European Molecular Biology Laboratory, EMBL Rome - Epigenetics and Neurobiology Unit, via E. Ramarini 32, 00015 Monterotondo, Italy.
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Polina V. Pavlovich
1European Molecular Biology Laboratory, EMBL Rome - Epigenetics and Neurobiology Unit, via E. Ramarini 32, 00015 Monterotondo, Italy.
2Max Planck Institute of Immunobiology and Epigenetics, Stübeweg 51, D-79108, Freiburg, Germany.
3Moscow Institute of Physics and Technology, Institutskii Per. 9, Moscow Region, Dolgoprudny 141700, Russia.
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Morgan Oatley
1European Molecular Biology Laboratory, EMBL Rome - Epigenetics and Neurobiology Unit, via E. Ramarini 32, 00015 Monterotondo, Italy.
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Kerstin Ganter
1European Molecular Biology Laboratory, EMBL Rome - Epigenetics and Neurobiology Unit, via E. Ramarini 32, 00015 Monterotondo, Italy.
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Rachel McKernan
1European Molecular Biology Laboratory, EMBL Rome - Epigenetics and Neurobiology Unit, via E. Ramarini 32, 00015 Monterotondo, Italy.
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Radvile Prialgauskaite
1European Molecular Biology Laboratory, EMBL Rome - Epigenetics and Neurobiology Unit, via E. Ramarini 32, 00015 Monterotondo, Italy.
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Artem Adamov
1European Molecular Biology Laboratory, EMBL Rome - Epigenetics and Neurobiology Unit, via E. Ramarini 32, 00015 Monterotondo, Italy.
3Moscow Institute of Physics and Technology, Institutskii Per. 9, Moscow Region, Dolgoprudny 141700, Russia.
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Konstantin Chukreev
1European Molecular Biology Laboratory, EMBL Rome - Epigenetics and Neurobiology Unit, via E. Ramarini 32, 00015 Monterotondo, Italy.
3Moscow Institute of Physics and Technology, Institutskii Per. 9, Moscow Region, Dolgoprudny 141700, Russia.
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Nicolas Descostes
1European Molecular Biology Laboratory, EMBL Rome - Epigenetics and Neurobiology Unit, via E. Ramarini 32, 00015 Monterotondo, Italy.
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Andreas Buness
1European Molecular Biology Laboratory, EMBL Rome - Epigenetics and Neurobiology Unit, via E. Ramarini 32, 00015 Monterotondo, Italy.
4Core Unit for Bioinformatics Data Analysis, Universitätsklinikum Bonn, Sigmund-Freud-Str. 25, 53127 Bonn, Germany
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Nina Cabezas-Wallscheid
2Max Planck Institute of Immunobiology and Epigenetics, Stübeweg 51, D-79108, Freiburg, Germany.
5CIBBS -Centre for Integrative Biological Signaling Studies, University of Freiburg, Germany
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Christophe Lancrin
1European Molecular Biology Laboratory, EMBL Rome - Epigenetics and Neurobiology Unit, via E. Ramarini 32, 00015 Monterotondo, Italy.
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  • For correspondence: christophe.lancrin@embl.it
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Abstract

The Yolk Sac (YS) and Aorta-Gonad-Mesonephros (AGM) are two major haematopoietic regions during embryonic development. Interestingly, AGM is the only one generating haematopoietic stem cells (HSCs). To identify the difference between AGM and YS, we compared them using single-cell RNA sequencing between 9.5 and 11.5 days of mouse embryonic development and identified cell populations using CONCLUS, a new computational tool. The AGM was the only one containing neurons and a specific mesenchymal population, while the YS major component was an epithelial population expressing liver marker genes. In addition, the YS contained a major endothelial population expressing Stab2, a hyaluronan receptor, also highly expressed by liver endothelium. We demonstrated that the YS haematopoietic potential was restricted to Stab2-negative cells and that ectopic expression of Stab2 could reduce blood cell formation from endothelium. Our results indicate that the AGM is a tissue more favourable to HSCs development than the YS because of its microenvironment and the nature of its endothelial cells.

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Posted September 19, 2019.
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Single-cell atlas of major haematopoietic tissues sheds light on blood cell formation from embryonic endothelium
Maya Shvartsman, Polina V. Pavlovich, Morgan Oatley, Kerstin Ganter, Rachel McKernan, Radvile Prialgauskaite, Artem Adamov, Konstantin Chukreev, Nicolas Descostes, Andreas Buness, Nina Cabezas-Wallscheid, Christophe Lancrin
bioRxiv 774547; doi: https://doi.org/10.1101/774547
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Single-cell atlas of major haematopoietic tissues sheds light on blood cell formation from embryonic endothelium
Maya Shvartsman, Polina V. Pavlovich, Morgan Oatley, Kerstin Ganter, Rachel McKernan, Radvile Prialgauskaite, Artem Adamov, Konstantin Chukreev, Nicolas Descostes, Andreas Buness, Nina Cabezas-Wallscheid, Christophe Lancrin
bioRxiv 774547; doi: https://doi.org/10.1101/774547

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