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The trajectory of gait development in mice

Shyam Akula, Katherine B. McCullough, Claire Weichselbaum, View ORCID ProfileJoseph D. Dougherty, View ORCID ProfileSusan E. Maloney
doi: https://doi.org/10.1101/774885
Shyam Akula
1Department of Psychiatry, Washington University School of Medicine, St. Louis, MO, USA
2Department of Genetics, Washington University School of Medicine, St. Louis, MO, USA
3Harvard-MIT MD/PhD Program, Harvard Medical School, Boston, MA, USA
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Katherine B. McCullough
1Department of Psychiatry, Washington University School of Medicine, St. Louis, MO, USA
2Department of Genetics, Washington University School of Medicine, St. Louis, MO, USA
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Claire Weichselbaum
1Department of Psychiatry, Washington University School of Medicine, St. Louis, MO, USA
2Department of Genetics, Washington University School of Medicine, St. Louis, MO, USA
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Joseph D. Dougherty
1Department of Psychiatry, Washington University School of Medicine, St. Louis, MO, USA
2Department of Genetics, Washington University School of Medicine, St. Louis, MO, USA
4Intellectual and Developmental Disabilities Research Center, Washington University School of Medicine, St. Louis, MO, USA
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Susan E. Maloney
1Department of Psychiatry, Washington University School of Medicine, St. Louis, MO, USA
4Intellectual and Developmental Disabilities Research Center, Washington University School of Medicine, St. Louis, MO, USA
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  • For correspondence: maloneys@wustl.edu
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Abstract

Gait irregularities are prevalent in neurodevelopmental diseases and disorders (NDDs). However, there is a paucity of information on gait phenotypes in the NDD experimental models. This is in part due to the lack of understanding of the normal developmental trajectory of gait maturation in the mouse. Using the DigiGait system, we have developed a quantitative, standardized, and reproducible assay of developmental gait metrics in commonly used mouse strains that can be added to the battery of mouse model phenotyping. With this assay, we characterized the trajectory of gait in the developing C57BL/6J and FVB/AntJ mouse lines. In both lines, maturation of mouse gait between P21 and P30 was best reflected by aspects of the stance phase of the stride. Additionally, in C57BL/6J mice, gait maturation was observed through how the paw is loaded and unloaded during the stance phase of a stride. In FVB/AntJ mice, gait maturity during this developmental window was best observed through the decreased variability in paw area on the belt during stance. Our results also underscore the importance of considering body length when interpreting gait metrics. Further, our results show that background strain needs to be considered when comparing gait across models. Overall, our results show that aspects of mouse gait development parallel a timeline of normal human gait development, such as the percent of stride that is stance phase and swing phase. This study may be used as a standard reference for developmental gait phenotyping of a variety of murine models, such as models of neurodevelopmental disease.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted September 19, 2019.
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The trajectory of gait development in mice
Shyam Akula, Katherine B. McCullough, Claire Weichselbaum, Joseph D. Dougherty, Susan E. Maloney
bioRxiv 774885; doi: https://doi.org/10.1101/774885
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The trajectory of gait development in mice
Shyam Akula, Katherine B. McCullough, Claire Weichselbaum, Joseph D. Dougherty, Susan E. Maloney
bioRxiv 774885; doi: https://doi.org/10.1101/774885

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