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An inducible genome editing system for plants

View ORCID ProfileXin Wang, View ORCID ProfileLingling Ye, View ORCID ProfileRobertas Ursache, View ORCID ProfileAri Pekka Mähönen
doi: https://doi.org/10.1101/779140
Xin Wang
1Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki 00014, Finland
2Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, and Viikki Plant Science Centre, University of Helsinki, Helsinki 00014, Finland
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  • ORCID record for Xin Wang
Lingling Ye
1Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki 00014, Finland
2Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, and Viikki Plant Science Centre, University of Helsinki, Helsinki 00014, Finland
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Robertas Ursache
3Department of Plant Molecular Biology, Biophore, Campus UNIL-Sorge, University of Lausanne CH-1015, Lausanne, Switzerland
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Ari Pekka Mähönen
1Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki 00014, Finland
2Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, and Viikki Plant Science Centre, University of Helsinki, Helsinki 00014, Finland
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  • For correspondence: AriPekka.Mahonen@helsinki.fi
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ABSTRACT

Conditional manipulation of gene expression is a key approach to investigating the primary function of a gene in a biological process. While conditional and cell-type specific overexpression systems exist for plants, there are currently no systems available to disable a gene completely and conditionally. Here, we present a novel tool with which target genes can be efficiently conditionally knocked out at any developmental stage. The target gene is manipulated using the CRISPR-Cas9 genome editing technology, and conditionality is achieved with the well-established estrogen-inducible XVE system. Target genes can also be knocked-out in a cell-type specific manner. Our tool is easy to construct and will be particularly useful for studying genes which have null-alleles that are non-viable or show strong developmental defects.

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Posted September 23, 2019.
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An inducible genome editing system for plants
Xin Wang, Lingling Ye, Robertas Ursache, Ari Pekka Mähönen
bioRxiv 779140; doi: https://doi.org/10.1101/779140
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An inducible genome editing system for plants
Xin Wang, Lingling Ye, Robertas Ursache, Ari Pekka Mähönen
bioRxiv 779140; doi: https://doi.org/10.1101/779140

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