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Closed mitosis requires local disassembly of the nuclear envelope

View ORCID ProfileGautam Dey, View ORCID ProfileSiân Culley, View ORCID ProfileScott Curran, View ORCID ProfileRicardo Henriques, View ORCID ProfileWanda Kukulski, View ORCID ProfileBuzz Baum
doi: https://doi.org/10.1101/779769
Gautam Dey
1MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
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  • For correspondence: g.dey@ucl.ac.uk b.baum@ucl.ac.uk
Siân Culley
1MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
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Scott Curran
2The Francis Crick Institute, 1 Midland Rd, London NW1 1AT, United Kingdom
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Ricardo Henriques
1MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
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Wanda Kukulski
3MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Francis Crick Ave, Cambridge CB2 0QH, United Kingdom
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Buzz Baum
1MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
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  • For correspondence: g.dey@ucl.ac.uk b.baum@ucl.ac.uk
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Abstract

At the end of mitosis, eukaryotic cells must segregate both copies of their replicated genome into two new nuclear compartments (1). They do this either by first dismantling and later reassembling the nuclear envelope in a so called “open mitosis”, or by reshaping an intact nucleus and then dividing into two in a “closed mitosis” (2, 3). However, while mitosis has been studied in a wide variety of eukaryotes for over a century (4), it is not known how the double membrane of the nuclear envelope is split into two at the end of a closed mitosis without compromising the impermeability of the nuclear compartment (5). In studying this problem in the fission yeast Schizosaccharomyces pombe, a classical model for closed mitosis (5), we use genetics, live cell imaging and electron tomography to show that nuclear fission is achieved via local disassembly of the nuclear envelope (NE) within the narrow bridge that links segregating daughter nuclei. In doing so, we identify a novel inner NE-localised protein Les1 that restricts the process of local NE breakdown (local NEB) to the bridge midzone and prevents the leakage of material from daughter nuclei. The mechanics of local NEB in a closed mitosis closely mirror those of NEB in open mitosis (3), revealing an unexpectedly deep conservation of nuclear remodelling mechanisms across diverse eukaryotes.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted September 23, 2019.
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Closed mitosis requires local disassembly of the nuclear envelope
Gautam Dey, Siân Culley, Scott Curran, Ricardo Henriques, Wanda Kukulski, Buzz Baum
bioRxiv 779769; doi: https://doi.org/10.1101/779769
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Closed mitosis requires local disassembly of the nuclear envelope
Gautam Dey, Siân Culley, Scott Curran, Ricardo Henriques, Wanda Kukulski, Buzz Baum
bioRxiv 779769; doi: https://doi.org/10.1101/779769

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